“毛茸茸”根提取物作为“绿色”合成银纳米粒子和医疗应用的来源,RSC Advances

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“毛茸茸”根提取物作为“绿色”合成银纳米粒子和医疗应用的来源
RSC Advances ( IF 3.9 ) Pub Date : 2020-10-27 , DOI: 10.1039/d0ra07784d
Natalia Kobylinska ₁ , Anatolij Shakhovsky ₂ , Olena Khainakova ₃ , Dmytro Klymchuk ₄ , Liliya Avdeeva ₅ , Yakiv Ratushnyak ₂ , Volodymyr Duplij ₂ , Nadiia Matvieieva ₂

Affiliation

该研究的重点是使用青蒿的“毛状”根培养物的提取物合成银纳米粒子 (AgNPs) 。和Artemisia annua L. 首次报道了操作参数如溶剂类型、提取温度、黄酮类化合物浓度和艾草“毛状”根提取物的还原能力对所得纳米颗粒的粒径和产量的影响. 从所研究的溶剂中，发现浓度为 70 vol% 的水-乙醇混合物最适合从所有“毛状”根培养物中提取黄酮类化合物。A. annua和A. tilesii中总黄酮含量“毛”根提取物分别高达 80.0 ± 0.9 和 108 ± 4.4 mg RuE/g DW。通过 UPLC-ESI-UHR-Qq-TOF-MS 分析确认黄酮类化合物的鉴定。Luteolin-7-β- D-吡喃葡萄糖苷、异鼠李素 3 - O-葡萄糖苷、黄芩素-7- O-葡萄糖苷酸、芹菜素-7- O提取物中含有-葡萄糖苷、槲皮素、谷甾醇、咖啡酰奎宁、没食子酸、绿原酸和咖啡酸。这些提取物表现出高还原活性。观察到有效尺寸为 5-100 nm 的球形、椭圆形和三角形纳米颗粒。TEM 数据显示，从不同根克隆的提取物中获得的 NP 形状存在很大差异。在使用乙醇提取物的情况下发现了聚集和不规则的 NP，主要是聚集的，大小为 10-50 nm。在使用在 80 °C 下获得的水提取物的情况下，AgNPs 的尺寸减小到 10-30 nm。生物合成的 AgNPs 在 400-450 nm 范围内显示出表面等离子共振。采用圆盘扩散法研究制备的 AgNPs 对革兰氏阳性（金黄色葡萄球菌）的抗菌活性。ATCC 25923 (F-49))、革兰氏阴性菌（绿脓杆菌ATCC 27853 (F-51)、大肠杆菌ATCC 25922 (F-50)）和白色念珠菌ATCC 88-653 菌株。发现与1 mM AgNO ₃溶液相比，纳米颗粒在某些情况下具有更大的抑制微生物生长的能力。获得的 AgNPs 胶体溶液在室温下在黑暗中稳定 12 个月。因此，青蒿和青蒿“毛”根提取物可用于获得具有生物活性的 AgNP 。

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‘Hairy’ root extracts as source for ‘green’ synthesis of silver nanoparticles and medical applications

The research was focused on the synthesis of silver nanoparticles (AgNPs) using extracts from the “hairy” root cultures of Artemisia tilesii Ledeb. and Artemisia annua L. The effect of operational parameters such as type of solvent, temperature of extraction, flavonoids concentration, and reducing power of the wormwood “hairy” root extracts on the particle size and yield of the resultant nanoparticles is reported for the first time. From the studied solvents, a water–ethanol mixture with a concentration of 70 vol% was found to be the best for the extraction of flavonoids from all “hairy” root cultures. The total flavonoid contents in A. annua and A. tilesii “hairy” root extracts were up to 80.0 ± 0.9 and 108 ± 4.4 mg RuE per g DW, respectively. Identification of flavonoids was confirmed by UPLC-ESI-UHR-Qq-TOF-MS analysis. Luteolin-7-β-D-glucopyranosid, isorhamnetin 3-O-glucoside, baicalein-7-O-glucuronide, apigenin-7-O-glucoside, quercetin, sitosterol, caffeoylquinic, galic, chlorogenic and caffeic acids were founded in the extracts. These extracts demonstrated the high reducing activities. Spherical, oval and triangular nanoparticles with effective sizes of 5–100 nm were observed. The TEM data revealed great differences in the shapes of NPs, obtained from the extracts from different root clones. The clustered and irregular NPs were found in the case of using ethanol extracts, mostly aggregated and having the size of 10–50 nm. The sizes of AgNPs decreased to 10–30 nm in the case of using aqueous extracts obtained at 80 °C. Biosynthesized AgNPs showed surface plasmon resonance in the range of 400–450 nm. The antimicrobial activity of the as-produced AgNPs was studied by disc diffusion method on Gram-positive (Staphylococcus aureus ATCC 25923 (F-49)), Gram-negative (Pseudomonas aeruginosa ATCC 27853 (F-51), Escherichia coli ATCC 25922 (F-50)) and Candida albicans ATCC 88-653 strains. It was found that the nanoparticles in some cases possessed the greater ability to inhibit microorganism growth compared to 1 mM AgNO₃ solution. The colloidal solutions of the obtained AgNPs were stable in the dark for 12 months at room temperature. Thus, the A. annua and A. tilesii “hairy” root extracts can be used for obtaining of bioactive AgNPs.

更新日期：2020-10-27

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