细菌衍生的植物保护代谢物2,4-二乙酰基间苯三酚：在抑制和亚抑制浓度下对细菌细胞的影响,Biomolecules

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细菌衍生的植物保护代谢物2,4-二乙酰基间苯三酚：在抑制和亚抑制浓度下对细菌细胞的影响
Biomolecules ( IF 4.8 ) Pub Date : 2020-12-25 , DOI: 10.3390/biom11010013
William T Julian ₁ , Anastasia V Vasilchenko _{1,

2} , Daniil D Shpindyuk ₁ , Darya V Poshvina ₁ , Alexey S Vasilchenko ₁

Affiliation

2,4-二乙酰基间苯三酚（2,4-DAPG）是一种众所周知的细菌次生代谢产物，但是其对细菌细胞的抑制和亚抑制作用机理仍知之甚少。使用荧光光谱研究了2,4-DAPG对模型细菌菌株的作用机理，发现抗生素的作用涉及膜通透性的快速增加，同时在营养贫乏的培养基中其活力降低。同时，在营养丰富的培养基中产生了数小时的抗菌作用。原子力显微镜显示，暴露于2,4-DAPG时大肠埃希菌的外膜具有时间依赖性，而金黄色葡萄球菌已经观察到细胞具有细胞内渗漏的迹象。另外，2,4-DAPG抑制成熟生物膜中金黄色葡萄球菌和大肠杆菌细菌细胞的代谢活性。观察到的抗生物膜活性的差异取决于抗生素的浓度。使用具有可诱导的生物发光的细菌生物传感器评估2，4-DAPG作用的细胞内靶标，其对应于DNA和蛋白质损伤。它无法记录来自任一传感器的任何正面响应。结果，认为2，4-DAPG的杀菌作用与细菌屏障结构的破坏有关。测试了2,4-二乙酰基间苯三酚对果胶杆菌群体感应介导过程的亚抑制作用胡萝卜素。发现亚抑制浓度的2,4-DAPG以剂量依赖的方式降低了胡萝卜假单胞菌中酰基高丝氨酸内酯的生物合成。进一步的研究表明，2,4-DAPG可抑制细菌的代谢活性而不影响其生存能力。

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Bacterial-Derived Plant Protection Metabolite 2,4-Diacetylphloroglucinol: Effects on Bacterial Cells at Inhibitory and Subinhibitory Concentrations

2,4-Diacetylphloroglucinol (2,4-DAPG) is a well-known bacterial secondary metabolite, however, its mechanism of inhibitory and subinhibitory action on bacterial cells is still poorly understood. The mechanism of 2,4-DAPG action on model bacterial strains was investigated using fluorescent spectroscopy and the action of the antibiotic was found to involve a rapid increase in membrane permeability that was accompanied by a reduction in its viability in nutrient-poor medium. At the same time, antibacterial action in nutrient-rich medium developed for several hours. Atomic force microscopy demonstrated time-dependent disturbances in the outer membrane of Escherichia coli when exposed to 2,4-DAPG, while Staphylococcus aureus cells have been visualized with signs of intracellular leakage. In addition, 2,4-DAPG inhibited the metabolic activity of S. aureus and E. coli bacterial cells in mature biofilms. Observed differences in the antibiofilm activity were dependent upon antibiotic concentration. The intracellular targets of the action of 2,4-DAPG were assessed using bacterial biosensors with inducible bioluminescence corresponding to DNA and protein damage. It was unable to register any positive response from either sensor. As a result, the bactericidal action of 2,4-DAPG is believed to be associated with the destruction of the bacterial barrier structures. The subinhibitory effect of 2,4-diacetylphloroglucinol was tested on quorum-sensing mediated processes in Pectobacterium carotovorum. Subinhibitory concentrations of 2,4-DAPG were found to lower the biosynthesis of acyl-homoserine lactones in P. carotovorum in a dose-dependent manner. Further investigation elucidated that 2,4-DAPG inhibits the metabolic activity of bacteria without affecting their viability.

更新日期：2020-12-25

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