Identification and Antihypertension Study of Novel Angiotensin I-Converting Enzyme Inhibitory Peptides from the Skirt of Chlamys farreri Fermented with Bacillus natto,Journal of Agricultural and Food Chemistry

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Identification and Antihypertension Study of Novel Angiotensin I-Converting Enzyme Inhibitory Peptides from the Skirt of Chlamys farreri Fermented with Bacillus natto
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-12-24 , DOI: 10.1021/acs.jafc.0c04232
Jie Gao _{1,

2} , Qi Liu ₂ , Ling Zhao ₂ , Jia Yu ₁ , Shanglong Wang ₃ , Tingfeng Cao ₃ , Xiang Gao ₁ , Yuxi Wei ₁

Affiliation

The aim of this study was to isolate the angiotensin I-converting enzyme (ACE) inhibitory peptides from the skirt of Chlamys farreri fermented with Bacillus natto and to explore the antihypertension effect through in vivo studies. ACE inhibitory peptides were purified from the fermentation mixture by ultrafiltration, gel filtration chromatography, and reversed-phase high-performance liquid chromatography sequentially. The amino acids’ sequence of the five novel ACE inhibitory peptides were identified by liquid chromatography–tandem mass spectrometry. Animal experiments demonstrated that the novel ACE inhibitory peptides significantly reduced the blood pressure in spontaneously hypertensive rats after a single or long-time treatment. Potential mechanisms were explored, and the results indicated that the novel peptides could regulate the renal renin-angiotensin system, improve vascular remodeling, inhibit myocardial fibrosis, and rebalance the gut microbial dysbiosis. Our results suggest that the fermentation products of the Chlamys farreri skirt by B. natto are potential sources of active peptides processing antihypertension activities.

中文翻译：

鉴定及抗高血压从研究的新裙子血管紧张素转换酶抑制肽栉孔扇贝发酵而成的纳豆芽孢杆菌

这项研究的目的是从纳豆芽孢杆菌发酵的蛤衣中分离出血管紧张素转换酶（ACE）抑制肽，并通过体内研究抗高血压作用学习。依次通过超滤，凝胶过滤色谱和反相高效液相色谱从发酵混合物中纯化ACE抑制肽。通过液相色谱-串联质谱法鉴定了五个新的ACE抑制肽的氨基酸序列。动物实验表明，经过一次或长期治疗后，新型ACE抑制肽可显着降低自发性高血压大鼠的血压。探索了潜在的机制，结果表明该新型肽可以调节肾素-血管紧张素系统，改善血管重塑，抑制心肌纤维化，并重新平衡肠道微生物失调。我们的结果表明，衣原体的发酵产物纳豆短裙是处理抗高血压活性的活性肽的潜在来源。

更新日期：2021-01-13

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