Synthetic dyes, extensively used in various industries, act as pollutants in the aquatic environment, and pose a significant threat to living beings. In the present study, we assessed the potential of a halophilic bacterium Salinivibrio kushneri HTSP isolated from a saltpan for decolorization and bioremediation of synthetic dyes. The genomic assessment of this strain revealed the presence of genes encoding the enzymes involved in decolorization mechanisms including FMN-dependent NADH azoreductase Clade III, which cleave the azo bond of the dye, and the enzymes involved in deamination and isomerization of intermediate compounds. The dye decolorization assay was performed using this bacterial strain on three water-soluble dyes in different concentrations: Coomassie brilliant blue (CBB) G-250 (500–3,000 mg/L), Safranin, and Congo red (50–800 mg/L). Within 48 h, more than 80% of decolorization was observed in all tested concentrations of CBB G-250 and Congo red dyes. The rate of decolorization was the highest for Congo red followed by CBB G-250 and then Safranin. Using UV-Visible spectrometer and Fourier Transform Infrared (FTIR) analysis, peaks were observed in the colored and decolorized solutions. The results indicated a breakdown of dyes upon decolorization, as some peaks were shifted and lost for different vibrations of aromatic rings, aliphatic groups (–CH₂, –CH₃) and functional groups (–NH, –SO₃H, and –SO₃⁻) in decolorized solutions. This study has shown the potential of S. kushneri HTSP to decolorize dyes in higher concentrations at a faster pace than previously reported bacterial strains. Thus, we propose that our isolated strain can be utilized as a potential dye decolorizer and biodegradative for wastewater treatment.

合成染料广泛应用于各个行业，它们在水生环境中充当污染物，对生物构成重大威胁。在本研究中，我们评估了嗜盐细菌的潜力库什内利弧菌从盐锅分离出的HTSP用于合成染料的脱色和生物修复。该菌株的基因组评估表明，存在着编码参与脱色机理的酶的基因，包括分裂染料的偶氮键的FMN依赖性NADH偶氮还原酶Clade III，以及参与中间化合物的脱氨和异构化的酶。使用该细菌菌株对三种不同浓度的水溶性染料进行了脱色试验：考马斯亮蓝（CBB）G-250（500–3,000 mg / L），番红花和刚果红（50–800 mg / L） ）。在48小时内，在所有测试浓度的CBB G-250和刚果红染料中均观察到超过80％的脱色。刚果红的脱色率最高，其次是CBB G-250，然后是番红花。使用紫外可见光谱仪和傅立叶变换红外（FTIR）分析，在有色和脱色溶液中观察到峰。结果表明，脱色后染料会分解，因为芳香族环，脂肪族基团（–CH₂，–CH ₃）和脱色溶液中的官能团（–NH，–SO ₃ H和–SO ₃ ⁻）。这项研究表明了库什尼HTSP可以以比以前报道的细菌菌株更快的速度使较高浓度的染料脱色。因此，我们建议我们分离出的菌株可以用作潜在的染料脱色剂和生物降解剂用于废水处理。