Pulsed Interleaved MINFLUX,Nano Letters

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Pulsed Interleaved MINFLUX
Nano Letters ( IF 9.6 ) Pub Date : 2020-12-18 , DOI: 10.1021/acs.nanolett.0c04600
Luciano A Masullo _{1,

2} , Florian Steiner ₃ , Jonas Zähringer ₃ , Lucía F Lopez ₁ , Johann Bohlen ₃ , Lars Richter _{1,

3} , Fiona Cole ₃ , Philip Tinnefeld ₃ , Fernando D Stefani _{1,

2}

Affiliation

We introduce p-MINFLUX, a new implementation of the highly photon-efficient single-molecule localization method with a simplified experimental setup and additional fluorescence lifetime information. In contrast to the original MINFLUX implementation, p-MINFLUX uses interleaved laser pulses to deliver the doughnut-shaped excitation foci at a maximum repetition rate. Using both static and dynamic DNA origami model systems, we demonstrate the performance of p-MINFLUX for single-molecule localization nanoscopy and tracking, respectively. p-MINFLUX delivers 1–2 nm localization precision with 2000–1000 photon counts. In addition, p-MINFLUX gives access to the fluorescence lifetime enabling multiplexing and super-resolved lifetime imaging. p-MINFLUX should help to unlock the full potential of innovative single-molecule localization schemes.

中文翻译：

脉冲交错式MINFLUX

我们介绍了p-MINFLUX，这是一种具有高光子效率的单分子定位方法的新实现，具有简化的实验设置和更多的荧光寿命信息。与最初的MINFLUX实施方案相反，p-MINFLUX使用交错的激光脉冲以最大重复率传递甜甜圈形激发焦点。使用静态和动态DNA折纸模型系统，我们分别证明了p-MINFLUX在单分子定位纳米显微镜和跟踪中的性能。p-MINFLUX具有2000–1000个光子计数，可实现1-2 nm的定位精度。此外，p-MINFLUX使荧光寿命得以实现，从而实现了多路复用和超分辨寿命成像。p-MINFLUX应该有助于释放创新的单分子定位方案的全部潜力。

更新日期：2021-01-13

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