Insight into the Deep Eutectic Solvent Extraction Mechanism of Flavonoids from Natural Plant,ACS Sustainable Chemistry & Engineering

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Insight into the Deep Eutectic Solvent Extraction Mechanism of Flavonoids from Natural Plant
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-12-18 , DOI: 10.1021/acssuschemeng.0c08146
Dan Cao ₁ , Qian Liu ₁ , Wenqiang Jing _{1,

2} , Haiyuan Tian ₁ , Hongyuan Yan ₃ , Wentao Bi ₁ , Yuliang Jiang ₁ , David Da Yong Chen _{1,

2}

Affiliation

Deep eutectic solvents (DESs) are a new class of green solvents with potential applications for the extraction of target compounds from both liquid and solid samples. However, current research in the field has focused on demonstrating the advantages in extraction efficiency in terms of more extracted material and shortened time, while the mechanism of the increased efficiency has not been systematically studied. Herein, we explored the solvent and solute interaction mechanisms with the use of three extraction methods (heating, microwave-assisted, and mechanochemical extraction) and different types of DESs. Choline chloride (ChCl) was used as the hydrogen-bond acceptor, while hydrogen-bond donors used are malonic acid, methylurea, and glycerin. Flavonoids from Flos Sophorae were extracted. ¹H nuclear magnetic resonance (NMR) spectrometry, ultraviolet–visible (UV–vis) spectrometry, scanning electron microscopy (SEM), and ultrahigh-performance liquid chromatography (UPLC) analyses were performed to investigate the interactions between the flavonoids and the plant cell walls with DESs, and chemical reactions between the DESs and flavonoids. We also systematically evaluated the influence of several key factors on the extraction efficiency, which was consistent with the experimental results. The influence of DES in the sample on qualitative and quantitative UPLC analyses was systematically studied, and conditions were optimized. This study should provide insights into the interactions of specific DESs with various target compounds and help design more efficient extraction methods.

中文翻译：

天然植物类黄酮的深共熔溶剂萃取机理研究

深共熔溶剂（DES）是一类新型的绿色溶剂，在从液体和固体样品中提取目标化合物方面都具有潜在的应用前景。然而，目前在该领域的研究集中于证明在提取效率方面的优势，即提取的材料更多，时间更短，而提高效率的机理尚未得到系统地研究。本文中，我们使用三种萃取方法（加热，微波辅助和机械化学萃取）和不同类型的DES探索了溶剂和溶质的相互作用机理。氯化胆碱（ChCl）被用作氢键受体，而氢键供体是丙二酸，甲基脲和甘油。从槐花中提取黄酮。^1个进行了H核磁共振（NMR）光谱，紫外-可见（UV-vis）光谱，扫描电子显微镜（SEM）和超高效液相色谱（UPLC）分析，以研究类黄酮与植物细胞壁之间的相互作用。与DES以及DES与类黄酮之间的化学反应。我们还系统地评估了几个关键因素对提取效率的影响，这与实验结果是一致的。系统研究了样品中DES对定性和定量UPLC分析的影响，并优化了条件。这项研究应提供有关特定DES与各种目标化合物相互作用的见解，并有助于设计更有效的提取方法。

更新日期：2020-12-28

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