电聚合受体涂层，用于基于导管的诊断传感器定量检测组胺,ACS Sensors

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电聚合受体涂层，用于基于导管的诊断传感器定量检测组胺
ACS Sensors ( IF 8.2 ) Pub Date : 2020-12-18 , DOI: 10.1021/acssensors.0c01844
Gideon Wackers ₁ , Peter Cornelis ₁ , Tristan Putzeys _{1,

2} , Marloes Peeters ₃ , Jan Tack ₄ , Freddy Troost ₅ , Theodor Doll ₆ , Nicolas Verhaert ₂ , Patrick Wagner ₁

Affiliation

在本文中，我们报告了基于导管的仿生传感器的发展，这是在功能性肠病情况下迈向微创诊断仪器的一步。组胺是小肠过敏和炎症过程的关键介质。然而，确定十二指肠粘膜处的组胺水平是一个挑战，经典的生物受体不适合在肠液的消化介质中使用。因此，我们开发了用于阻抗感应电极的分子印迹聚吡咯涂层，该涂层能够定量检测浓度范围从25 nM到1μM的未稀释的人肠液中的组胺。电极对作为竞争分子的组氨酸的交叉敏感性可忽略不计，为进行比较，我们还评估了非印迹和牛磺酸印迹的聚吡咯对组胺的反应。此外，使用等效电路模型，我们发现聚吡咯对组胺的分子识别主要增加了电极-液体界面的电阻成分，而电容效应可忽略不计。集成在导管中的传感器进行差分测量，以校正肠液复杂基质中的非特异性吸附作用，并且单个触发频率足以确定组胺浓度。这些功能一起对实时诊断测试很有帮助。我们发现聚吡咯对组胺的分子识别主要增加了电极-液体界面的电阻成分，而电容效应却可以忽略不计。集成在导管中的传感器进行差分测量，以校正肠液复杂基质中的非特异性吸附作用，并且单个触发频率足以确定组胺浓度。这些功能一起对实时诊断测试很有帮助。我们发现聚吡咯对组胺的分子识别主要增加了电极-液体界面的电阻成分，而电容效应却可以忽略不计。集成在导管中的传感器进行差分测量，以校正肠液复杂基质中的非特异性吸附作用，并且单个触发频率足以确定组胺浓度。这些功能一起对实时诊断测试很有帮助。

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Electropolymerized Receptor Coatings for the Quantitative Detection of Histamine with a Catheter-Based, Diagnostic Sensor

In this article, we report on the development of a catheter-based, biomimetic sensor as a step toward a minimally invasive diagnostic instrument in the context of functional bowel disorders. Histamine is a key mediator in allergic and inflammatory processes in the small intestines; however, it is a challenge to determine histamine levels at the duodenal mucosa, and classical bioreceptors are unsuitable for use in the digestive medium of bowel fluid. Therefore, we have developed molecularly imprinted polypyrrole coatings for impedimetric sensing electrodes, which enable the quantification of histamine in nondiluted, human bowel fluid in a broad concentration range from 25 nM to 1 μM. The electrodes show negligible cross-sensitivity to histidine as a competitor molecule and, for comparison, we also evaluated the response of nonimprinted and taurine-imprinted polypyrrole to histamine. Furthermore, using equivalent-circuit modeling, we found that the molecular recognition of histamine by polypyrrole primarily increases the resistive component of the electrode–liquid interface while capacitive effects are negligible. The sensor, integrated into a catheter, measures differentially to correct for nonspecific adsorption effects in the complex matrix of bowel fluids, and a single triggering frequency is sufficient to determine histamine concentrations. Together, these features are beneficial for real-time diagnostic tests.

更新日期：2021-01-22

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