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Perylene Dianhydride and Perylene Diimide Luminophores Integrated with Gold Nanoparticles for Dual-Potential Electrochemiluminescence Ratiometric Immunosensors
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2020-12-17 , DOI: 10.1021/acsanm.0c02981 Wei Zhang 1 , Yue Song 1 , Yunyun Wang 1 , Yingchao Gong 1 , Lei Shang 1 , Rongna Ma 1 , Liping Jia 1 , Qingwang Xue 1 , Yuchang Du 2 , Shuijian He 3 , Huaisheng Wang 1
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2020-12-17 , DOI: 10.1021/acsanm.0c02981 Wei Zhang 1 , Yue Song 1 , Yunyun Wang 1 , Yingchao Gong 1 , Lei Shang 1 , Rongna Ma 1 , Liping Jia 1 , Qingwang Xue 1 , Yuchang Du 2 , Shuijian He 3 , Huaisheng Wang 1
Affiliation
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In electrochemiluminescence (ECL) field, screening luminophores, especially potential-resolved luminophores with the same parent chromophore, is essential for the development of fundamental theory study and accurate assay application such as ratiometric assay in biomedical research and clinical diagnosis. In this work, we developed a luminophore, reported a pair of potential-resolved luminophores with perylene as chromophore, and fabricated a dual-potential ECL ratiometric immunosensor. Luminophore chlorine-substituted perylene dianhydride (PTCDA-Cl4) was reported to produce strong ECL at −1.2 V with a wavelength at 630 nm. The ECL mechanism was interpreted with excited J-type PTCDA-Cl4 dimers. PTCDA-Cl4 and perylene diimide exhibited potential-resolved ECL features with K2S2O8 as a coreactant between 0 and −1.2 V, which were used to integrate gold nanoparticles for fabricating dual-potential ECL ratiometric immunosensors to detect α-fetoprotein (AFP). The biosensor showed good linear response for AFP from 10 fg mL–1 to 100 pg mL–1 with a detection limit of 3.33 fg mL–1. The biosensor also showed good application in real sample detection.
中文翻译：
Gold二酐和Per二酰亚胺发光体与金纳米粒子集成，用于双电势电化学发光比例免疫传感器。
在电化学发光（ECL）领域中，筛选发光体，尤其是具有相同亲本发色团的电位分辨发光体，对于基础理论研究和精确测定应用（例如生物医学研究和临床诊断中的比率测定）的发展至关重要。在这项工作中，我们开发了一种发光体，报告了一对以per为生色团的电势分辨发光体，并制造了一种双电势ECL比例免疫传感器。据报道，荧光团氯取代的ylene二酐（PTCDA-Cl 4）在-1.2 V时产生强ECL，波长为630 nm。用激发的J型PTCDA-Cl 4二聚体解释了ECL机制。PTCDA-Cl 4per二酰亚胺显示出电位分辨的ECL特征，其中K 2 S 2 O 8作为共活化剂在0至-1.2 V之间，用于整合金纳米颗粒，以制备双电位ECL比例免疫传感器以检测甲胎蛋白（AFP）。从10 fg mL –1到100 pg mL –1，生物传感器对AFP表现出良好的线性响应，检出限为3.33 fg mL –1。该生物传感器在实际样品检测中也显示出良好的应用。
更新日期：2021-01-22
中文翻译：
Gold二酐和Per二酰亚胺发光体与金纳米粒子集成，用于双电势电化学发光比例免疫传感器。
在电化学发光（ECL）领域中，筛选发光体，尤其是具有相同亲本发色团的电位分辨发光体，对于基础理论研究和精确测定应用（例如生物医学研究和临床诊断中的比率测定）的发展至关重要。在这项工作中，我们开发了一种发光体，报告了一对以per为生色团的电势分辨发光体，并制造了一种双电势ECL比例免疫传感器。据报道，荧光团氯取代的ylene二酐（PTCDA-Cl 4）在-1.2 V时产生强ECL，波长为630 nm。用激发的J型PTCDA-Cl 4二聚体解释了ECL机制。PTCDA-Cl 4per二酰亚胺显示出电位分辨的ECL特征，其中K 2 S 2 O 8作为共活化剂在0至-1.2 V之间，用于整合金纳米颗粒，以制备双电位ECL比例免疫传感器以检测甲胎蛋白（AFP）。从10 fg mL –1到100 pg mL –1，生物传感器对AFP表现出良好的线性响应，检出限为3.33 fg mL –1。该生物传感器在实际样品检测中也显示出良好的应用。
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