当前位置：
X-MOL 学术
›
J. Nat. Prod.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Role of Ascaridole and p-Cymene in the Sleep-Promoting Effects of Dysphania ambrosioides Essential Oil via the GABAergic System in a ddY Mouse Inhalation Model
Journal of Natural Products ( IF 3.3 ) Pub Date : 2020-12-16 , DOI: 10.1021/acs.jnatprod.0c01137 Godfried Dougnon 1 , Michiho Ito 1
Journal of Natural Products ( IF 3.3 ) Pub Date : 2020-12-16 , DOI: 10.1021/acs.jnatprod.0c01137 Godfried Dougnon 1 , Michiho Ito 1
Affiliation
|
The essential oil obtained from Dysphania ambrosioides leaves (DAEO) has antifungal, antioxidant, and antimicrobial properties. This study investigated DAEO’s chemical composition and its sleep-promoting effects via administration by inhalation in ddY mice. Ascaridole (35.5%) and p-cymene (47.2%) were the major components. To obtain insight into DAEO’s effects on the central nervous system (CNS), ascaridole and p-cymene were evaluated for sedative activity by using the caffeine-treated excitatory mouse model. DAEO administration significantly decreased locomotor activity at all doses except 0.000 04 mg per 400 μL of triethyl citrate. Both ascaridole and p-cymene were highly effective in decreasing locomotor activity of excited mice by more than 50%. In addition, ascaridole and p-cymene prolonged the pentobarbital-induced sleeping duration by 42% and 77%, respectively. These effects were antagonized by coadministration of gamma-aminobutyric acid (GABAA)-benzodiazepine receptor antagonist, flumazenil (3 mg/kg), indicating that the GABAergic system mediates the sedative effect. Finally, inhaled ascaridole and p-cymene had no negative effect on motor coordination, as observed during the Rota-rod test. Therefore, via activation of the GABAergic system, ascaridole and p-cymene mediate the sleep-promoting effect of DAEO. The results further extend the knowledge on their use as potential promising natural products for the management of sleep disorders and CNS-related ailments.
中文翻译：
Ascaridole 和 p-Cymene 在 DYY 小鼠吸入模型中通过 GABA 系统促进 Dysphania Ambrosioides 精油的睡眠作用
从Dysphania ambrosioides叶 (DAEO) 中提取的精油具有抗真菌、抗氧化和抗菌特性。本研究通过在 ddY 小鼠中通过吸入给药来研究 DAEO 的化学成分及其促进睡眠的作用。Ascaridole (35.5%) 和p- cymene (47.2%) 是主要成分。为了深入了解 DAEO 对中枢神经系统 (CNS) 的影响，通过使用咖啡因处理的兴奋性小鼠模型评估蛔虫和对伞花烃的镇静活性。除了每 400 μL 柠檬酸三乙酯 0.000 04 mg 外，DAEO 给药显着降低所有剂量的运动活动。蛔虫和p-cymene 能非常有效地将兴奋小鼠的运动活动降低 50% 以上。此外，蛔虫和对伞花烃使戊巴比妥诱导的睡眠时间分别延长了 42% 和 77%。这些作用被γ-氨基丁酸（GABA A）-苯二氮卓受体拮抗剂氟马西尼（3 mg/kg）的共同给药所拮抗，表明GABA能系统介导镇静作用。最后，正如在 Rota-rod 测试中所观察到的，吸入蛔虫和对伞花烃对运动协调没有负面影响。因此，通过激活 GABA 能系统，蛔虫和p-cymene 介导 DAEO 的睡眠促进作用。结果进一步扩展了将它们用作治疗睡眠障碍和中枢神经系统相关疾病的潜在有前途的天然产品的知识。
更新日期：2021-01-22
中文翻译：
Ascaridole 和 p-Cymene 在 DYY 小鼠吸入模型中通过 GABA 系统促进 Dysphania Ambrosioides 精油的睡眠作用
从Dysphania ambrosioides叶 (DAEO) 中提取的精油具有抗真菌、抗氧化和抗菌特性。本研究通过在 ddY 小鼠中通过吸入给药来研究 DAEO 的化学成分及其促进睡眠的作用。Ascaridole (35.5%) 和p- cymene (47.2%) 是主要成分。为了深入了解 DAEO 对中枢神经系统 (CNS) 的影响，通过使用咖啡因处理的兴奋性小鼠模型评估蛔虫和对伞花烃的镇静活性。除了每 400 μL 柠檬酸三乙酯 0.000 04 mg 外，DAEO 给药显着降低所有剂量的运动活动。蛔虫和p-cymene 能非常有效地将兴奋小鼠的运动活动降低 50% 以上。此外，蛔虫和对伞花烃使戊巴比妥诱导的睡眠时间分别延长了 42% 和 77%。这些作用被γ-氨基丁酸（GABA A）-苯二氮卓受体拮抗剂氟马西尼（3 mg/kg）的共同给药所拮抗，表明GABA能系统介导镇静作用。最后，正如在 Rota-rod 测试中所观察到的，吸入蛔虫和对伞花烃对运动协调没有负面影响。因此，通过激活 GABA 能系统，蛔虫和p-cymene 介导 DAEO 的睡眠促进作用。结果进一步扩展了将它们用作治疗睡眠障碍和中枢神经系统相关疾病的潜在有前途的天然产品的知识。
京公网安备 11010802027423号