1. Microsatellite genotyping is an important genetic method for a number of research questions in biology. Given that the traditional fragment length analysis using polyacrylamide gel or capillary electrophoresis has several drawbacks, microsatellite genotyping‐by‐sequencing (GBS) has arisen as a promising alternative. Although GBS mitigates many of the problems of fragment length analysis, issues with allelic dropout and null alleles often remain due to mismatches in primer binding sites and unnecessarily long PCR products. This is also true for GBS in catarrhine primates where cross‐species amplification of loci (often human derived) is common.
2. We therefore redesigned primers for 45 microsatellite loci based on 17 available catarrhine reference genomes. Next, we tested them in singleplex and different multiplex settings in a panel of species representing all major lineages of Catarrhini and further validated them in wild Guinea baboons (Papio papio) using fecal samples.
3. The final panel of 42 microsatellite loci can efficiently be amplified with primers distributed into three amplification pools.
4. With our microsatellite panel, we provide a tool to universally genotype catarrhine primates via GBS from different sample sources in a cost‐ and time‐efficient way, with higher resolution, and comparability among laboratories and species.

中文翻译：

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精制的42个微卫星基因座，适用于通用基因型卡他汀灵长类动物

1. 微卫星基因分型是生物学中许多研究问题的重要遗传方法。鉴于使用聚丙烯酰胺凝胶或毛细管电泳进行的传统片段长度分析存在若干缺点，因此微卫星基因分型测序（GBS）已成为一种有前途的替代方法。尽管GBS减轻了片段长度分析的许多问题，但由于引物结合位点的错配和不必要的较长PCR产物，等位基因缺失和无效等位基因的问题通常仍然存在。对于卡他灵长类动物中GBS的情况也是如此，那里的物种（通常是人类衍生的）基因座跨物种扩增很常见。
2. 因此，我们基于17个可用的卡他汀参考基因组重新设计了45个微卫星基因座的引物。接下来，我们在代表Catarrhini所有主要谱系的一组物种中以单重和不同多重设置对它们进行了测试，并使用粪便样品在野生几内亚狒狒（Papio papio）中对其进行了进一步验证。
3. 最终的42个微卫星基因座可以用分布在三个扩增池中的引物有效地扩增。
4. 借助我们的微卫星面板，我们提供了一种工具，可通过GBS以成本和时间高效的方式通过GBS通用基因型卡他汀灵长类动物，具有更高的分辨率以及实验室和物种之间的可比性。