In Drosophila melanogaster, ecdysis-triggering hormone (ETH) and eclosion hormone (EH) mutually enhance the release of each other through a positive feedback loop. Both are involved in the stimulation of ecdysis behavior series and molting-related physiological preparations. However, whether 20-hydroxyecdysone (20E) controls eh expression and whether EH plays a similar role in non-Drosophilid insects remain to be determined. In Leptinotarsa decemlineata, we found that Ldeh mRNA levels were high in the early and/or late stages and low in the intermediate period within each larval instar, indicating that the mRNA peaks positively correlate with 20E pulses. Consistent with the indication, we confirmed that 20E signaling stimulated the transcription of Ldeh. RNA interference (RNAi)-aided knockdown of Ldeh at the final instar stage slightly impaired pupation and significantly affected wing expansion. Around 20% of the Ldeh RNAi larvae remained as prepupae, completely wrapped in the old larval cuticles. These prepupae gradually darkened, dried and withered, and finally died. The remaining (about 80%) Ldeh hypomorphs became pupae and emerged as abnormal adults, bearing smaller and wrinkle elytra and hindwings. The adult tracheae in the Ldeh hypomorphs were full of liquid. In addition, the low pupation rate and high deformed adult proportion cannot be rescued by ingestion of either 20E or juvenile hormone. Thus, EH is critical for ecdysis and trachea clearance in L. decemlineata. The failure of trachea clearance may disenable air-swallowing during larva-pupa-adult transition and thus impacts wing growth and expansion.

在果蝇中，促蜕变激素（ETH）和促分泌激素（EH）通过正反馈回路相互促进彼此的释放。两者都参与刺激蜕皮行为系列和蜕皮相关的生理准备。然而，是否20-羟基蜕皮激素（20E）是否控制eh表达，以及EH是否在非果蝇昆虫中起类似作用尚待确定。在Leptinotarsa decemlineata中，我们发现Ldeh每个幼虫龄期的mRNA水平在早期和/或晚期均较高，而在中期则较低，这表明mRNA峰值与20E脉冲呈正相关。与适应症一致，我们证实了20E信号传导刺激了Ldeh的转录。在最后的成年期，RNA干扰（RNAi）辅助的Ldeh敲低会严重影响化脓，并严重影响机翼的扩张。Ldeh RNAi幼虫中约有20％保留为原虫，完全包裹在旧的幼虫表皮中。这些足p逐渐变黑，干燥，枯萎，最后死亡。其余（约80％）Ldeh亚体变p，并以不正常的成虫形式出现，具有较小的皱纹鞘翅和后翅。Ldeh次体中的成年气管充满液体。另外，化脓率低和成年人畸形比例高不能通过摄入20E或少年激素来挽救。因此，EH对于落叶松（L. decemlineata）的蜕皮和气管清除至关重要。气管清除失败可能会阻止幼虫-pu成虫过渡期间的空气吞咽，从而影响机翼的生长和扩张。