Novel pyrene- and anthracene-based Schiff base derivatives (A1 and P1) were designed and prepared for selective “turn-on” fluorescence determination of Hg²⁺ ions in food and environmental samples. Structural characterization of novel compounds was performed by standard spectroscopic methods and photophysical properties were exanimated by UV–vis, steady-state fluorescence, time-resolved fluorescence, 3D-fluorescence and excitation-emission matrix (EEM) analysis. Investigation of binding and interaction mechanism clearly indicated that A1 and P1 can sensitively and selectively detect Hg²⁺ ions in real samples via inhibition of PET processes after 1:1 complex formation. In order to perform the determination of Hg²⁺ ions in food and environmental samples, experimental conditions such as pH, buffer, sensor concentration and measurement time were optimized. The detection limits of presented methods were determined as 12.22 nM (2.45 μg L⁻¹) and 8.32 nM (1.67 μg L⁻¹) in the linear range of 0.005 μM–45 μM and 0.008 μM–38 μM for A1 and P1, respectively. After validation with ICP-MS and spike/recovery test, A1 and P1 were successfully used for fluorescence analysis of Hg²⁺ ions in food and environmental samples. In addition, the paper-based test kit was prepared with pyrene appended Schiff base (P1) which, could be applied successfully for monitoring Hg²⁺ ions.

设计并制备了基于pyr和蒽的新型Schiff碱衍生物（A1和P1），用于选择性“开启”荧光测定食品和环境样品中的Hg ²⁺离子。新化合物的结构表征是通过标准光谱方法进行的，光物理性质通过紫外可见，稳态荧光，时间分辨荧光，3D荧光和激发发射矩阵（EEM）分析得到证实。对结合和相互作用机制的研究清楚地表明，A1和P1可以灵敏和选择性地检测Hg ²⁺1：1配合物形成后，通过抑制PET过程，使真实样品中的离子释放出来。为了测定食品和环境样品中的Hg ²⁺离子，优化了诸如pH，缓冲液，传感器浓度和测量时间等实验条件。的提出的方法的检测限确定为12.22纳米（2.45微克大号^-1）和8.32纳米（1.67微克大号^-1在0.005μM-45μM和0.008μM-38μM为线性范围）A1和P1，分别。经过ICP-MS验证和加标/恢复测试后，A1和P1成功用于Hg ^2+的荧光分析食物和环境样品中的离子。此外，该纸质测试试剂盒还配有pyr基席夫碱（P1），可成功用于监测Hg ²⁺离子。