The difficulties in the process of cellulose cascade conversion based on immobilization technology lies in the recycling enzymes from rich solid-containing straw hydrolysate and the incompatibility of conventional immobilization with this process. In this study, three types of enzyme (cellulase, glucose oxidase and catalase) were successfully immobilized on a reversible soluble Eudragit L-100. Through the determination of the preparation conditions, enzymatic properties and catalytic conditions, the co-immobilized enzyme was applied to the catalytic reaction of one-pot conversion of corn straw to gluconic acid. The yield of gluconic acid achieved 0.28 mg/mg, conversion rate of cellulose in corn straw to gluconic acid reached 61.41%. The recovery of co-immobilized enzyme from solid substrate was achieved by using reversible and soluble characteristics of the carrier. After 6 times of recycling, the activity of co-immobilized enzyme was maintained at 52.38%, confirming the feasibility of multi-enzyme immobilization strategy using reversible soluble carrier in cascade reactions.

中文翻译：

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在级联催化中将多酶共固定在可逆溶性聚合物上，用于玉米秸秆中葡萄糖酸的一锅转化


基于固定化技术的纤维素梯级转化过程中的难点在于从富含固体的秸秆水解物中回收酶，以及常规固定化与该工艺的不相容性。在这项研究中，三种类型的酶（纤维素酶、葡萄糖氧化酶和过氧化氢酶）被成功固定在可逆可溶性 Eudragit L-100 上。通过测定制备条件、酶性能和催化条件，将共固定化酶应用于玉米秸秆一锅转化葡萄糖酸的催化反应。葡萄糖酸得率达到 0.28 mg/mg，玉米秸秆中纤维素向葡萄糖酸的转化率达到 61.41%。利用载体的可逆和可溶特性，从固体底物中回收共固定化酶。经过 6 次循环，共固定化酶的活性保持在 52.38%，证实了在级联反应中使用可逆可溶性载体进行多酶固定化策略的可行性。