Honey and curcumin were widely used both in modern and ancient medicines. The present research work focuses on developing an antibacterial, antioxidant, biocompatible, biodegradable, non-immunogenic, and completely natural, electrospun nanofibrous wound healing membrane using curcumin and Indian honey collected from different regions of Tamilnadu, belonging to the same species. A nanofibrous membrane was fabricated using the combination of Honey, Curcumin, and Gelatin. The fabricated membrane was analyzed using Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM) which showed the surface morphology and the average diameter of 159.08 ± 53.88 nm of the nanofiber. The antioxidant property of the membrane was studied through scavenging the free radicals of 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power (FRAP), and Phosphomolybdenum assays. The developed membrane showed 500% swelling and the contact angle studies showed the surface wettability of the nanofiber. The membrane was also found to be cytocompatible, enhancing adhesion and proliferation using cell proliferation studies. The in-vitro wound scratch studies showed that the wound closure happened by 18 h when treated with L929 mouse fibroblast cells, justified using the excision wound model. In-vitro kinetic studies showed a maximum release of both honey and curcumin achieved in 32 h for the membrane. The In-vivo wound healing studies demonstrated that the Honey and Curcumin loaded nanofibrous membrane indeed showed a better wound contraction of 89.05 ± 0.47% in comparison with other samples tested.

蜂蜜和姜黄素广泛用于现代和古代医学中。目前的研究工作集中在使用姜黄素和印度蜂蜜中提取的一种抗菌，抗氧化剂，生物相容性，可生物降解，非免疫原性和完全天然的电纺纳米纤维伤口愈合膜，该蜂蜜来自印度泰米尔纳德邦不同地区，属于同一物种。使用蜂蜜，姜黄素和明胶的组合制备了纳米纤维膜。使用傅立叶变换红外光谱法（FTIR），扫描电子显微镜（SEM）分析制成的膜，其显示出纳米纤维的表面形态和平均直径为159.08±53.88nm。通过清除2,2-二苯基-1-吡啶并肼基（DPPH）2的自由基，研究了膜的抗氧化性能。体外伤口刮擦研究表明，使用切除的伤口模型证明，当用L929小鼠成纤维细胞处理伤口闭合时，伤口闭合发生了18小时。体外动力学研究表明，蜂蜜和姜黄素的最大释放量在32小时内达到了膜的水平。的在体内伤口愈合表明，蜂蜜和姜黄素装载纳米纤维膜学习的确显示出89.05±0.47％更好的伤口收缩与测试其它样品比较。