Tris(2,3-dibromopropyl) isocyanurate (TBC) is widely used in polymer products. It is ubiquitously found in environmental matrices. Previous studies have shown that TBC has potential endocrine-disrupting effects on aquatic organisms. However, the effects on adrenocortical function and the underlying mechanisms are not well characterized. In present study, the H295R cell line was employed to investigate the potential endocrine-disrupting action of TBC. TBC inhibited basal and forskolin-induced sex hormone production but did not exhibit cytotoxicity. The expression of the steroidogenic genes, StAR , 3βHSD2 , CYP17 , CYP21 , CYP19 , HMGR , 17βHSD1 , 17βHSD4 and CYP11A , was not changed upon TBC exposure. However, the cAMP inducer forskolin strongly induced the expression of all these genes, except for HMGR , 17βHSD1 and 17βHSD4 . TBC blocked forskolin-induced StAR , 3βHSD2 , CYP17 , CYP21 and CYP19 mRNA expression. Our results indicate that TBC can inhibit sex hormone biosynthesis due to the interference with steroidogenic gene transcription in activated condition.

中文翻译：

---

三（2,3-二溴丙基）异氰脲酸酯对H295R细胞类固醇生成的影响

三（2,3-二溴丙基）异氰脲酸酯（TBC）被广泛用于聚合物产品中。它在环境矩阵中无处不在。先前的研究表明，TBC对水生生物具有潜在的内分泌干扰作用。然而，对肾上腺皮质功能及其潜在机制的影响尚不十分清楚。在本研究中，H295R细胞系用于研究TBC的潜在内分泌干扰作用。TBC抑制基础和福斯高林诱导的性激素产生，但不表现出细胞毒性。类固醇生成基因 StAR ， 3βHSD2 ， CYP17 ， CYP21 ， CYP19 ， HMGR ， 17βHSD1的表达 ， 在暴露于TBC时未改变 17βHSD4 和 CYP11A 。然而，除了 HMGR ， 17βHSD1 和 17βHSD4 之外，cAMP诱导剂毛喉素强烈诱导了所有这些基因的表达 。TBC阻断了佛司可林诱导的 StAR ， 3βHSD2 ， CYP17 ， CYP21 和 CYP19 mRNA的表达。我们的结果表明，TBC可以在激活条件下抑制类固醇生成基因的转录，从而抑制性激素的生物合成。