水稻中DWARF3的一个新的等位基因突变体的表征，并在存在Strigolactone的情况下分析其功能和稳定性,Molecular Breeding

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水稻中DWARF3的一个新的等位基因突变体的表征，并在存在Strigolactone的情况下分析其功能和稳定性
Molecular Breeding ( IF 2.6 ) Pub Date : 2017-03-11 , DOI: 10.1007/s11032-017-0640-x
Yueyang Liang , Shiquan Wang , Xiaoxi Huang , Haipeng Wang , Fenlong Liu , Shuangcheng Li , Jun Zhu , Qiming Deng , Huainian Liu , Aiping Zheng , Lingxia Wang , Ping Li

Strigolactones（SLs）是重要的内在生长调节剂，可通过协调芽和根的发育来控制植物的结构。最近的研究表明SL信号通过靶向伴侣蛋白样蛋白的降解蛋白DWARF53（D53）家族起作用。此过程需要DWARF14（D14）作为马来酸内酯信号受体，并且需要DWARF3（D3）作为泛素E3连接酶形成Skp-Cullin-F-box（SCF）复合物。尽管可以预期这些信号成分的相互作用，但尚不确定SLs信号在何处以及如何以组织特异性方式在细胞内发生。在这项研究中，我们表征了水稻高分矮化突变体 ext.-M1B ，表现出对合成的strigolactone混合物 rac-GR24的抗性 。通过基因分析，我们发现 ext.-M1B 是 D3的 一个新的等位基因突变体，具有核苷酸突变，导致截短的宽型D3蛋白。我们证明，该突变既不影响基因表达水平也不影响蛋白质亚细胞定位，而它破坏了 ext.-M1B 突变体中SL信号的感知。此外，我们发现在野生型背景中 D3的 过表达不会引起明显的表型，但会抑制 D3 RNA干扰产生的结果清晰显示了SL突变体的表型。通过在水稻中表达荧光D3融合蛋白，我们首先表明，无论是否经过strigolactone处理，D3在核中始终稳定。两者合计，我们的数据表明，D3在核中编码F-box蛋白，作为对稻草内酯调节稻芽结构的稳定信号成分的响应。

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Characterization of a new allelic mutant of DWARF3 in rice and analysing its function and stability in the presence of strigolactone

Strigolactones (SLs) are important intrinsic growth regulators that control plant architecture by coordinating shoot and root development. Recent studies demonstrate that SL signals act via targeting the degradation protein DWARF53 (D53) family of chaperonin-like proteins. This process requires DWARF14 (D14) as strigolactones signal receptor and DWARF3 (D3) forming Skp-Cullin-F-box (SCF) complex as ubiquitin E3 ligase. Although the interactions of these signal components can be expected, where and how the SLs signalling occur within cells in a tissue-specific manner is still uncertain. In this study, we characterize a rice high-tillering dwarf mutant, ext.-M1B , displaying resistance to synthetic strigolactone mixture rac-GR24 . Through genetic analysis, we find that ext.-M1B is a new allelic mutant of D3 with a nucleotide mutation resulting in a truncated protein of wide-type D3. We demonstrate that the mutation affects neither gene expression level nor the protein sub-cellular localization, whereas it disrupts the perception of SLs signal in ext.-M1B mutant. Moreover, we find that overexpression of D3 in wild type background causes no significant phenotype, but suppression of D3 by RNA interfering results in a clear phenocopy of SL mutants. By expressing fluorescent D3 fusion protein in rice, we first show that D3 is stable consistently in the nucleus with or without strigolactone treatment. Taken together, our data indicates that D3 encoding an F-box protein in nucleus, as a stable signal component response to strigolactone regulating rice shoot architecture.

更新日期：2017-03-11

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