Solanum sisymbriifolium, a wild relative of the S. lycopersicum species, has been found to be resistant to numerous pathogens which cause severe diseases in Solanaceae crops. It would be highly desirable for species strongly affected by diseases (e.g. tomato) to contain the resistant genes found in their wild relatives. Solanum sisymbriifolium has been considered of potential interest for S. melongena L. breeding, as the former is resistant to several pathogens and resistant interspecific hybrids have been obtained. Additionally, several reports indicate that S. sisymbriifolium is useful for tomato gynogenic haploid production. It is still not quite clear whether S. sisymbriifolium can be crossed with S. lycopersicum and the development of hybrid progeny is possible. In our preliminary in vivo crossing, S. lycopersicum × S. sisymbriifolium diploid embryos were formed inside fruits, but their development was inhibited at the globular stage. To obtain F1 hybrids the embryo-rescue method was implemented. Globular embryos isolated 14–35 dap were cultured on various media showing varied morphogenic potencies. Most of the embryos did not develop on the used media but calli formed from the embryogenic cells in > 17% of the embryos, allowing hybrid plants to be obtained. Ten regenerants, which were adapted in pots containing soil, had morphological traits similar to the S. sisymbriifolium parent, including the plant habit, presence of prickles on shoots or white colour of flowers. The hybrid origin of the regenerants was confirmed by flow cytometry analysis of DNA content and KASP genotyping. The results indicated that S. lycopersicum can be hybridized with S. sisymbriifolium through interspecific hybridization to introduce novel traits for use in tomato-breeding programmes.

中文翻译：

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Solanum lycopersicum L. 和 S. sisymbriifolium Lam 种间杂种的发展。通过胚胎愈伤组织

Solanum sisymbriifolium 是 S. lycopersicum 物种的野生近缘种，已被发现对导致茄科作物严重疾病的多种病原体具有抗性。非常希望受疾病严重影响的物种（例如番茄）含有在其野生近缘种中发现的抗性基因。Solanum sisymbriifolium 被认为对 S. melongena L. 育种具有潜在意义，因为前者对几种病原体具有抗性，并且已经获得了抗性种间杂种。此外，一些报告表明 S. sisymbriifolium 可用于番茄雌性单倍体生产。S. sisymbriifolium 是否可以与S. lycopersicum 杂交以及开发杂交后代是否可能还不是很清楚。在我们初步的体内杂交中，S. lycopersicum × S. sisymbriifolium二倍体胚在果实内部形成，但在球状阶段发育受到抑制。为了获得 F1 杂种，实施了胚胎拯救方法。分离 14-35 dap 的球状胚胎在各种培养基上培养，显示出不同的形态发生能力。大多数胚胎没有在使用的培养基上发育，而是由超过 17% 的胚胎中的胚胎发生细胞形成愈伤组织，从而获得杂交植物。在装有土壤的盆中适应的 10 种再生子具有与 S. sisymbriifolium 亲本相似的形态特征，包括植物习性、枝条上的刺或花的白色。再生体的杂交来源通过 DNA 含量的流式细胞术分析和 KASP 基因分型得到证实。结果表明 S. lycopersicum 可以与 S. lycopersicum 杂交。