A liquid chromatographic method was applied to determine trifolirhizin, kushenol K, kushenol L, kushenol N, kushenol X, kurarinone, norkurarinone, isokurarinone and kushenol A in the roots of Sophora flavescens, namely Kushen in China. The samples were separated on a YMC-C18 column (250 × 4.6 mm, 5 μm) with a gradient of methanol and 0.3% aqueous acetic acid (v/v) at a flow rate of 0.8 mL min−1 and detected at 295 nm. The complete separation was achieved within 45 min for the nine major flavonoids. All calibration curves expressed good linearity (r2 > 0.999) within the test range. The recovery of this method was 92.3–106.9%. The assay was successfully applied to the quantification of nine flavonoids in 26 samples of Kushen. The results indicated that this developed LC assay could be readily utilized as a quality control method for the Chinese herb medicine Kushen.

中文翻译：

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反相液相色谱法同时测定苦参中九种主要黄酮类化合物

应用液相色谱法测定苦参根中苦参根中的苦参素、苦参素K、苦参酚L、苦参酚N、苦参酚X、苦参酮、去甲苦参酮、异苦参酮和苦参酚A。样品在 YMC-C18 柱（250 × 4.6 mm，5 μm）上用甲醇和 0.3% 醋酸水溶液 (v/v) 梯度分离，流速为 0.8 mL min - 1，并在 295 nm 处检测9 种主要黄酮类化合物在 45 分钟内实现完全分离。所有校准曲线在测试范围内都表现出良好的线性 (r2> 0.999)。该方法的回收率为92.3-106.9%。该方法成功应用于苦参26份样品中9种黄酮类化合物的定量分析。