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Electrogenerated Chemiluminescence Immunoassays on Nanoelectrode Ensembles Platform with Magnetic Microbeads for the Determination of Carbohydrate Antigen
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-03 , DOI: 10.1021/acs.analchem.0c03047 Xiaolin Yang 1 , Yuxi Wei 1 , Yujin Du 1 , Honglan Qi 1 , Qiang Gao 1 , Chengxiao Zhang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-03 , DOI: 10.1021/acs.analchem.0c03047 Xiaolin Yang 1 , Yuxi Wei 1 , Yujin Du 1 , Honglan Qi 1 , Qiang Gao 1 , Chengxiao Zhang 1
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This work reports a gold nanoelectrode ensembles (Au-NEE) platform taken as a disposable electrogenerated chemiluminescence (ECL) platform with immunomagnetic microbeads for ECL immunoassays for the first time. The peak-shaped voltammograms were obtained at the Au-NEE, attributed to the total diffusional overlap. The ECL intensity at Au-NEE was 12.9 folds in the Ru(bpy)32+-tri-n-propylamine (TPA) ECL system and 19.6 folds in the luminol-H2O2 system, compared with that at the Au macroelectrode using the normalized active area of the electrodes, mainly attributed to the diffusion overlap of the Au-NEE and the edge effect of the individual gold nanodisks of the Au-NEE. The ECL immunoassay on the Au-NEE platform with magnetic microbeads for the determination of cancer biomarkers was developed. Carbohydrate antigen 19-9 (CA 19-9) was chosen as a model analyte while CA 19-9 antibody on the magnetic microbeads was taken as the capture probe, and ruthenium complex-labeled CA 19-9 antibody was used as the signal probe. A “sandwich” bioconjugates on the magnetic beads were transferred onto the ECL platform, and then the ECL measurements were performed in TPA solution. The developed method showed that the ECL peak intensity was directly in proportion to the concentration of CA 19-9 in the range from 0.5 to 20 U/mL with a limit of detection of 0.4 U/mL. This work demonstrates that the Au-NEE can be employed as a useful disposable ECL platform with the merits of cheapness, low nonspecific adsorption and practical application. The proposed approach will open a new avenue in the point-of-care test for the determination of protein biomarkers.
中文翻译：
磁性微珠纳米电极集成平台上的电化学发光免疫分析法测定碳水化合物抗原
这项工作首次报道了金纳米电极集成（Au-NEE）平台作为带有免疫磁性微珠的一次性电生成化学发光（ECL）平台，用于ECL免疫测定。归因于总扩散重叠，在Au-NEE处获得了峰形伏安图。在Ru（bpy）3 2 + -三-正丙胺（TPA）ECL系统中，Au-NEE的ECL强度为12.9倍，在鲁米诺-H 2 O 2中为19.6倍与使用电极的归一化有效面积的Au宏电极相比，该系统主要归因于Au-NEE的扩散重叠和Au-NEE的各个金纳米盘的边缘效应。开发了具有磁性微珠的Au-NEE平台上的ECL免疫测定法，用于测定癌症生物标志物。选择碳水化合物抗原19-9（CA 19-9）作为模型分析物，同时将磁性微珠上的CA 19-9抗体用作捕获探针，并使用钌络合物标记的CA 19-9抗体作为信号探针。将磁珠上的“三明治”生物共轭物转移到ECL平台上，然后在TPA溶液中进行ECL测量。所开发的方法表明，ECL峰强度与CA 19-9的浓度成正比，范围为0.5至20 U / mL，检出限为0.4 U / mL。这项工作表明，Au-NEE具有便宜，非特异性吸附低和实际应用的优点，可以用作有用的一次性ECL平台。所提出的方法将为确定蛋白质生物标志物的即时检验开辟新途径。
更新日期：2020-12-15
中文翻译：
磁性微珠纳米电极集成平台上的电化学发光免疫分析法测定碳水化合物抗原
这项工作首次报道了金纳米电极集成（Au-NEE）平台作为带有免疫磁性微珠的一次性电生成化学发光（ECL）平台，用于ECL免疫测定。归因于总扩散重叠，在Au-NEE处获得了峰形伏安图。在Ru（bpy）3 2 + -三-正丙胺（TPA）ECL系统中，Au-NEE的ECL强度为12.9倍，在鲁米诺-H 2 O 2中为19.6倍与使用电极的归一化有效面积的Au宏电极相比，该系统主要归因于Au-NEE的扩散重叠和Au-NEE的各个金纳米盘的边缘效应。开发了具有磁性微珠的Au-NEE平台上的ECL免疫测定法，用于测定癌症生物标志物。选择碳水化合物抗原19-9（CA 19-9）作为模型分析物，同时将磁性微珠上的CA 19-9抗体用作捕获探针，并使用钌络合物标记的CA 19-9抗体作为信号探针。将磁珠上的“三明治”生物共轭物转移到ECL平台上，然后在TPA溶液中进行ECL测量。所开发的方法表明，ECL峰强度与CA 19-9的浓度成正比，范围为0.5至20 U / mL，检出限为0.4 U / mL。这项工作表明，Au-NEE具有便宜，非特异性吸附低和实际应用的优点，可以用作有用的一次性ECL平台。所提出的方法将为确定蛋白质生物标志物的即时检验开辟新途径。
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