Chemical Labeling of Protein 4′‐Phosphopantetheinylation,ChemBioChem

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Chemical Labeling of Protein 4′‐Phosphopantetheinylation
ChemBioChem ( IF 2.6 ) Pub Date : 2020-12-01 , DOI: 10.1002/cbic.202000747
Nan Chen ₁ , Chu Wang _{1,

2}

Affiliation

Structurally diverse chemical probes have been used to label protein 4′‐phosphopantetheinylation. Herein, we review how CoA analogs could be incorporated by highly promiscuous phosphopantetheinyl transferases (PPTases) and used for in situ labeling of the membrane proteins fused with acceptor tags. These cell‐permeable pantetheine analogs facilitated the metabolic labeling of the endogenous substrates in both bacterial and mammalian cells.

中文翻译：

蛋白质 4'-磷酸酯酰化的化学标记

结构多样的化学探针已被用于标记蛋白质 4'-磷酸泛酰巯基乙胺化。在此，我们回顾了 CoA 类似物如何被高度混杂的磷酸泛酰巯基乙胺转移酶 (PPTases) 掺入并用于原位标记与受体标签融合的膜蛋白。这些细胞可渗透的泛酰巯基乙胺类似物促进了细菌和哺乳动物细胞中内源性底物的代谢标记。

更新日期：2020-12-01

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