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DNAzyme-powered DNA walking machine for ultrasensitive fluorescence aptasensing of kanamycin
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-11-28 , DOI: 10.1007/s00604-020-04638-4 Zongqi Yang 1 , Mei Liu 1 , Baoxin Li 2
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-11-28 , DOI: 10.1007/s00604-020-04638-4 Zongqi Yang 1 , Mei Liu 1 , Baoxin Li 2
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A DNAzyme-powered DNA walking machine was constructed to develop the fluorescence aptasensing for sensitive detection of kanamycin. The aptamer for kanamycin is partially hybridized with complementary DNA (cDNA) modified on magnetic beads (MBs). The specific interaction of target and aptamer triggered the cDNA to be free tentatively, which captured walker DNA. Then the autonomous motion of DNA walker on MBs surface was propelled via DNAzyme digestion of recognition sites. The signal probe was separated, and the amplified fluorescence signal was achieved by the accumulation of the signal probe. Kanamycin was used as a model analyte, and the developed assay achieves a detection limit of 0.00039 ng·mL−1 (S/N = 3) within a linear detection range from 0.001 to 2000 ng·mL−1. This aptasensing strategy can be extended for detection of other antibiotics by adapting corresponding target recognition aptamer sequence. Graphical abstract The fluorescence aptasensing for sensitive detection of kanamycin based on DNAzyme-powered DNA walking machine was constructed. Graphical abstract The fluorescence aptasensing for sensitive detection of kanamycin based on DNAzyme-powered DNA walking machine was constructed.
中文翻译：
用于卡那霉素超灵敏荧光适配的 DNAzyme 驱动的 DNA 步行机
构建了一个 DNAzyme 驱动的 DNA 步行机，以开发用于灵敏检测卡那霉素的荧光适配传感。卡那霉素适体与在磁珠 (MB) 上修饰的互补 DNA (cDNA) 部分杂交。靶标和适体的特定相互作用触发了 cDNA 暂时游离，从而捕获了沃克 DNA。然后通过识别位点的 DNAzyme 消化推动 DNA walker 在 MBs 表面的自主运动。分离信号探针，通过信号探针的积累实现放大的荧光信号。卡那霉素用作模型分析物，开发的检测方法在 0.001 至 2000 ng·mL-1 的线性检测范围内实现了 0.00039 ng·mL-1 (S/N = 3) 的检测限。通过调整相应的目标识别适体序列，这种适体传感策略可以扩展到检测其他抗生素。图形摘要 构建了基于DNAzyme 驱动的DNA 行走机的卡那霉素灵敏检测荧光适配体。图形摘要 构建了基于DNAzyme 驱动的DNA 行走机的卡那霉素灵敏检测荧光适配体。
更新日期：2020-11-28
中文翻译：
用于卡那霉素超灵敏荧光适配的 DNAzyme 驱动的 DNA 步行机
构建了一个 DNAzyme 驱动的 DNA 步行机，以开发用于灵敏检测卡那霉素的荧光适配传感。卡那霉素适体与在磁珠 (MB) 上修饰的互补 DNA (cDNA) 部分杂交。靶标和适体的特定相互作用触发了 cDNA 暂时游离，从而捕获了沃克 DNA。然后通过识别位点的 DNAzyme 消化推动 DNA walker 在 MBs 表面的自主运动。分离信号探针，通过信号探针的积累实现放大的荧光信号。卡那霉素用作模型分析物，开发的检测方法在 0.001 至 2000 ng·mL-1 的线性检测范围内实现了 0.00039 ng·mL-1 (S/N = 3) 的检测限。通过调整相应的目标识别适体序列，这种适体传感策略可以扩展到检测其他抗生素。图形摘要 构建了基于DNAzyme 驱动的DNA 行走机的卡那霉素灵敏检测荧光适配体。图形摘要 构建了基于DNAzyme 驱动的DNA 行走机的卡那霉素灵敏检测荧光适配体。
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