Cercospora coffeicola is the causal agent of brown eye spot, an important disease of coffee (Coffea arabica) in Brazil. However, atypical symptom as darker and larger lesions, named black spot, has been reported in field. In this study, we tested the hypothesis that the causal agent of black spot belongs to the same species pathogenic to brown eye spot. Nineteen strains obtained from diseased coffee found in the five largest coffee-producing states of Brazil were characterized by a combination of molecular phylogenic methods, using a multi-locus approach (internal transcribed spacer regions and intervening 5.8S nrRNAs, actin, calmodulin, histone H3, and translation elongation factor 1-alpha), analyses of morphological markers and pathogenicity. Strains from brown eye spot and black spot disease on coffee leaves formed a clade with C. coffeicola strain from Japan. All strains showed same morphological characteristics and caused brown eye spot symptoms in greenhouse. These results confirm that the species associated with brown eye spot and black spot disease on coffee leaves is C. coffeicola. Additionally, an epitype is proposed for C. coffeicola.

Cercospora coffeicola是褐斑病的病原体，褐斑病是巴西的一种重要的咖啡病（阿拉伯咖啡）。然而，在野外已经报道了非典型症状，即较深和较大的病灶，称为黑斑。在这项研究中，我们检验了以下假设：黑斑病的病原体属于与棕色眼斑病原相同的物种。在巴西五个最大的咖啡生产州发现的十九种从患病咖啡中获得的菌株，通过分子系统学方法的组合，采用多基因座方法（内部转录间隔区和居间的5.8S nrRNA，肌动蛋白，钙调蛋白，组蛋白H3）进行了表征和翻译延伸因子1-alpha），形态标记和致病性分析。来自咖啡叶上的褐斑病和黑斑病的菌株与日本产的C.coffeicola菌株形成了进化枝。所有菌株均表现出相同的形态特征，并在温室中引起褐斑病症状。这些结果证实了与咖啡叶上的棕色眼斑和黑斑病有关的物种是C.coffeicola。另外，提出了咖啡果念珠菌的表型。