Fe–N–C single-atom nanozymes with peroxidase-like activity for the detection of alkaline phosphatase,Analyst

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Fe–N–C single-atom nanozymes with peroxidase-like activity for the detection of alkaline phosphatase
Analyst ( IF 3.6 ) Pub Date : 2020-11-13 , DOI: 10.1039/d0an01846e
Xiaolei Xie _{1,

2,

3,

4} , Yufei Wang _{2,

4,

5,

6} , Xiaobin Zhou _{1,

2,

3,

4} , Junyang Chen _{1,

2,

3,

4} , Mengke Wang _{1,

2,

3,

4} , Xingguang Su _{1,

2,

3,

4}

Affiliation

Single-atom nanozymes have drawn wide attention in bio-sensing for their remarkable merits such as low cost, high stability, and maximum atom utilization. Herein, a colorimetric strategy based on Fe–N–C single-atom nanozymes (Fe/NC-SAs) was established for the detection of alkaline phosphatase (ALP) activity. The Fe/NC-SAs prepared by pyrolysis have excellent peroxidase-like activity and can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue color product in the presence of hydrogen peroxide (H₂O₂). When ascorbic acid (AA) is added to the system, the blue color fades, and the absorbance has a linear relationship with the concentration of AA. Alkaline phosphatase (ALP) can catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) to produce AA. Thus, a strategy based on Fe/NC-SAs for the detection of ALP activity was established, which provided a linear range of 0.1–1.5 U L⁻¹ and a limit of detection as low as 0.05 U L⁻¹. Besides, Fe/NC-SAs showed high stability under harsh conditions. Moreover, an Fe/NC-SA-based assay was successfully validated using human serum samples for ALP determination with satisfactory results, and has broad prospects in the field of biosensing.

中文翻译：

具有过氧化物酶样活性的Fe–N–C单原子纳米酶，用于检测碱性磷酸酶

单原子纳米酶以其低成本，高稳定性和最大的原子利用率等显着优点而在生物传感领域引起了广泛关注。在这里，建立了基于Fe–N–C单原子纳米酶（Fe / NC-SAs）的比色策略，用于检测碱性磷酸酶（ALP）活性。通过热解制备的Fe / NC-SA具有优异的过氧化物酶样活性，在过氧化氢（H ₂ O _2）存在下可将3,3'，5,5'-四甲基联苯胺（TMB）氧化为蓝色产物）。将抗坏血酸（AA）添加到系统中时，蓝色褪色，并且吸光度与AA浓度呈线性关系。碱性磷酸酶（ALP）可以催化抗坏血酸2-磷酸（AAP）水解生成AA。因此，建立了基于Fe / NC-SAs的ALP活性检测策略，该策略提供了0.1–1.5 UL ^-1的线性范围和低至0.05 UL ^-1的检测限。此外，Fe / NC-SAs在恶劣条件下也显示出高稳定性。此外，基于Fe / NC-SA的测定法已成功使用人血清样品进行ALP测定，结果令人满意，在生物传感领域具有广阔的前景。

更新日期：2020-11-25

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