Acidic compounds were enriched from a water decoction of Portulaca oleracea using 717 anion exchange resin column chromatography. A total of 22 compounds including 9 catecholamine derivatives, of which six were rare sulfonic acid derivatives, and 9 nitro derivatives, were further isolated through various column chromatographic methods, and their structures were elucidated by interpreting their spectroscopic data and ECD calculations. Among them, 16 compounds were isolated from P. oleracea for the first time, 8 of which were undescribed compounds and four compounds were natural products. Pharmacological screening indicated that cis-3-(3-nitro-4-hydroxyphenyl)-methyl acrylate exhibited anti-inflammatory activity, measured as inhibition of nitric oxide production in LPS-stimulated RAW264.7 macrophage cells, with an EC50 value of 18.0 μM, The compounds showed only weak anti-microbial activity with (2R)-(+)-2-chloro-3-(3-nitro-4-hydroxyphenyl)-propionic acid methyl ester inhibiting Candida albicans with a MIC of 256 μg/mL, and 3-methoxy-4,5-dinitrophenol inhibiting Shigella sonnei with a MIC of 512 μg/mL.

中文翻译：

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马齿苋儿茶酚类生物碱磺酸盐和芳香硝基化合物及其抗炎抗微生物活性筛选

使用 717 阴离子交换树脂柱色谱法从马齿苋的水煎剂中富集酸性化合物。通过各种柱色谱方法进一步分离了包括9种儿茶酚胺衍生物在内的22种化合物，其中6种为稀有磺酸衍生物，9种硝基衍生物，并通过光谱数据解释和ECD计算阐明了它们的结构。其中，16个化合物是首次从P. oleracea中分离得到，其中8个化合物为未描述化合物，4个化合物为天然产物。药理学筛选表明，顺式-3-(3-硝基-4-羟基苯基)-甲基丙烯酸酯表现出抗炎活性，测量为抑制 LPS 刺激的 RAW264.7 巨噬细胞中的一氧化氮产生，EC50 值为 18.0 μM ,