当前位置: X-MOL 学术ACS Sustain. Chem. Eng. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Metal–Organic Framework as a Compartmentalized Integrated Nanozyme Reactor to Enable High-Performance Cascade Reactions for Glucose Detection
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-11-23 , DOI: 10.1021/acssuschemeng.0c06325
Xiqing Cheng 1 , Zhiping Zheng 1 , Xirong Zhou 1 , Qin Kuang 1
Affiliation  

Integrated nanozymes have recently attracted intense attention because of their promising applications in many fields, including biosensing. However, methods to efficiently activate cascade reactions remain challenging when designing and constructing integrated nanozymes. Herein, inspired by the compartmentalization and substrate-channeling effects of natural enzymes in organisms, a metal–organic framework (MOF)-based integrated nanozyme was prepared by encapsulating a natural enzyme (GOx) within the copper 1,4-benzenedicarboxylate (CuBDC) MOF nanozyme via a one-step biomimetic mineralization process at room temperature. Benefiting from the highly ordered peroxidase-like active sites in CuBDC and the confinement effect of CuBDC, the compartmentalization and substrate-channeling effects were achieved in the GOx@CuBDC integrated nanozyme. Surprisingly, the GOx@CuBDC integrated nanozyme displayed a 12.5-fold enhancement compared with the cascade reaction system using free GOx and CuBDC in detecting glucose. Furthermore, this biosensor exhibited a good linear relationship in the concentration range 10–500 μM, with a low detection limit of 4.1 μM (S/N = 3), and also demonstrated excellent stability, reusability, and selectivity. This work may spark a new design strategy for high-performance integrated nanozymes.

中文翻译:

金属有机框架作为分隔的集成纳米酶反应器,可实现高性能级联反应,用于葡萄糖检测

集成纳米酶由于其在包括生物传感在内的许多领域中的广阔应用前景,最近引起了广泛关注。然而,当设计和构建整合的纳米酶时,有效激活级联反应的方法仍然具有挑战性。在此,受自然酶在生物体内的分隔和底物通道效应的启发,通过将天然酶(GOx)封装在1,4-苯二甲酸铜(CuBDC)中,制备了基于金属-有机框架(MOF)的集成纳米酶MOF纳米酶通过一步仿生矿化过程在室温下进行。受益于CuBDC中高度有序的过氧化物酶样活性位点和CuBDC的封闭效应,在GOx @ CuBDC集成纳米酶中实现了区室化和底物通道效应。令人惊讶的是,与使用游离GOx和CuBDC的级联反应系统检测葡萄糖相比,GOx @ CuBDC集成的纳米酶显示出12.5倍的增强。此外,该生物传感器在10–500μM的浓度范围内表现出良好的线性关系,检测限低至4.1μM(S / N = 3),并且还具有出色的稳定性,可重复使用性和选择性。这项工作可能会为高性能的集成纳米酶激发新的设计策略。和选择性。这项工作可能会为高性能的集成纳米酶激发新的设计策略。和选择性。这项工作可能会为高性能的集成纳米酶激发新的设计策略。
更新日期:2020-11-23
down
wechat
bug