确定用于猪体外成熟的最佳卵母细胞辅助抽吸条件：对卵丘卵母细胞复合物与卵母细胞质量之间关系的重新评估,Veterinary Medicine and Science

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确定用于猪体外成熟的最佳卵母细胞辅助抽吸条件：对卵丘卵母细胞复合物与卵母细胞质量之间关系的重新评估
Veterinary Medicine and Science ( IF 1.8 ) Pub Date : 2020-11-21 , DOI: 10.1002/vms3.378
Giuseppe Silvestri ₁ , Claudia C. Rathje ₁ , Simon C. Harvey ₂ , Rebecca L. Gould ₃ , Grant A. Walling ₄ , Peter J.I. Ellis ₁ , Katie E. Harvey ₂ , Darren K. Griffin ₁

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用于IVF的猪卵母细胞的质量通常根据卵母细胞周围卵丘细胞（CC）的层数进行分级。这些一起形成卵母细胞复合体（COC）。CC的至少三个紧凑层被认为对有效的IVP很重要。为了测试这一点，根据卵丘投资对卵母细胞进行了评分，其中A级代表具有三层或更多层卵丘的COC，包括颗粒细胞卵丘卵母细胞复合体，B级代表完整日冕放射线被另一层卵丘细胞包围的C级，C级和C级。 D代表CC投资较低的COC。然后监测这些卵母细胞的体外成熟度（IVM），通过微管蛋白免疫染色评估减数分裂的进展，皮质颗粒环的发育以及谷胱甘肽水平。结果表明，分级与核成熟和细胞质成熟密切相关，表明通过卵丘投资对卵母细胞进行分级是预测IVM成功的可靠方法。重要的是，A级和B级卵母细胞在任何方面均无显着差异，因此使用两个或更多个CC层作为截止可能是最佳选择。我们还确定了辅助抽吸对卵母细胞取回的影响，比较了针头大小和施加的压力对取卵率的影响。这些数据表明，这两个变量都影响卵母细胞的回收率和回收卵母细胞的质量。综合起来，这些实验表明，A级和B级卵母细胞具有相似的发育潜力，并且使用18号针头和50 mmHg的抽吸压力可使这些级卵母细胞的回收率最大化。

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Identification of optimal assisted aspiration conditions of oocytes for use in porcine in vitro maturation: A re‐evaluation of the relationship between the cumulus oocyte complex and oocyte quality

The quality of porcine oocytes for use in IVF is commonly graded according to the number of layers of cumulus cells (CCs) surrounding the oocyte; together these form the cumulus oocyte complex (COC). At least three compact layers of CCs is regarded as important for efficient IVP. To test this, oocytes were scored according to cumulus investment, with grade A representing COCs with three or more cumulus layers including granulosa cell‐cumulus oocyte complexes, grade B those with an intact corona radiata surrounded by another layer of cumulus cells and grades C and D representing COCs with lower CC investment. These oocytes were then monitored for in vitro maturation (IVM), as assessed by tubulin immunostaining for meiotic progression, the development of a cortical granule ring, and by glutathione levels. Results indicate that grading correlates closely with nuclear maturation and cytoplasmic maturation, suggesting that grading oocytes by cumulus investment is a reliable method to predict IVM success. Importantly, Grade A and B oocytes showed no significant differences in any measure and hence using a cut‐off of two or more CC layers may be optimal. We also determined the effect of assisted aspiration for oocyte retrieval, comparing the effect of needle size and applied pressure on the retrieval rate. These data indicated that both variables affected oocyte recovery rates and the quality of recovered oocytes. In combination, these experiments indicate that grade A and B oocytes have a similar developmental potential and that the recovery of oocytes of these grades is maximised by use of an 18‐gauge needle and 50 mmHg aspiration pressure.

更新日期：2020-11-21

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