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Carbon Nanohorns/Pt Nanoparticles/DNA Nanoplatform for Intracellular Zn2+ Imaging and Enhanced Cooperative Phototherapy of Cancer Cells
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-11-20 , DOI: 10.1021/acs.analchem.0c03880 Fuzhi Shen 1 , Caiyi Zhang 2 , Zhiheng Cai 1 , Jiwei Wang 1 , Xing Zhang 3 , Jeremiah Ong’achwa Machuki 1 , Hengliang Shi 1, 4 , Fenglei Gao 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-11-20 , DOI: 10.1021/acs.analchem.0c03880 Fuzhi Shen 1 , Caiyi Zhang 2 , Zhiheng Cai 1 , Jiwei Wang 1 , Xing Zhang 3 , Jeremiah Ong’achwa Machuki 1 , Hengliang Shi 1, 4 , Fenglei Gao 1
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Superfluous zinc ion (Zn2+) in living cells has been identified as a potential tumor biomarker for early cancer diagnosis and cancer progression monitoring. In this paper, we developed a novel carbon nanohorns/Pt nanoparticles/DNA (CNHs/Pt NPs/DNA) nanoplatform based on the clamped hybridization chain reaction (c-HCR) process for intracellular Zn2+ imaging and enhanced cooperative phototherapy of cancer cells. Cross-shaped DNAzyme (c-DNAzyme), hairpin DNA1, hairpin DNA2, and aptamer DNA were adsorbed onto the surfaces of CNHs/Pt NPs, and the fluorescence of carboxytetramethyl-rhodamine was also quenched. After entering the living cells, the c-DNAzyme was cleaved to output trigger DNA in the existence of intracellular Zn2+ and initiate the c-HCR process for fluorescence amplification. Compared with the single HCR process triggered by a single DNAzyme, the c-HCR process could further improve the amplification efficiency and sensitivity. In addition, such a nanoprobe possesses a catalysis-enhanced photodynamic effect by Pt NP generation of oxygen in a tumor microenvironment and increases the photothermal effect by loading of Pt NPs on CNHs, indicating that this is a promising biological method for cancer diagnosis and cancer cell therapy.
中文翻译：
碳纳米角/ Pt纳米颗粒/ DNA纳米平台用于细胞内Zn 2+成像和癌细胞的协同协同光疗
活细胞中多余的锌离子（Zn 2+）已被确定为早期癌症诊断和癌症进展监测的潜在肿瘤生物标志物。在本文中，我们基于用于细胞内Zn 2+成像的钳位杂交链反应（c-HCR）过程和增强的癌细胞协同光疗技术，开发了一种新型的碳纳米角/铂纳米颗粒/ DNA（CNHs /铂纳米颗粒/ DNA）纳米平台。。十字形DNA酶（c-DNAzyme），发夹DNA1，发夹DNA2和适体DNA吸附在CNHs / Pt NPs的表面上，羧四甲基罗丹明的荧光也被淬灭。进入活细胞后，在细胞内存在Zn 2+的情况下，裂解c-DNAzyme以输出触发DNA。并启动c-HCR过程进行荧光扩增。与单个DNAzyme触发的单个HCR过程相比，c-HCR过程可以进一步提高扩增效率和灵敏度。此外，这种纳米探针通过在肿瘤微环境中产生氧的Pt NP具有催化增强的光动力效应，并通过在CNH上负载Pt NP来增加光热效应，表明这是一种有前途的生物学方法，可用于癌症诊断和癌细胞诊断治疗。
更新日期：2020-12-15
中文翻译：
碳纳米角/ Pt纳米颗粒/ DNA纳米平台用于细胞内Zn 2+成像和癌细胞的协同协同光疗
活细胞中多余的锌离子（Zn 2+）已被确定为早期癌症诊断和癌症进展监测的潜在肿瘤生物标志物。在本文中，我们基于用于细胞内Zn 2+成像的钳位杂交链反应（c-HCR）过程和增强的癌细胞协同光疗技术，开发了一种新型的碳纳米角/铂纳米颗粒/ DNA（CNHs /铂纳米颗粒/ DNA）纳米平台。。十字形DNA酶（c-DNAzyme），发夹DNA1，发夹DNA2和适体DNA吸附在CNHs / Pt NPs的表面上，羧四甲基罗丹明的荧光也被淬灭。进入活细胞后，在细胞内存在Zn 2+的情况下，裂解c-DNAzyme以输出触发DNA。并启动c-HCR过程进行荧光扩增。与单个DNAzyme触发的单个HCR过程相比，c-HCR过程可以进一步提高扩增效率和灵敏度。此外，这种纳米探针通过在肿瘤微环境中产生氧的Pt NP具有催化增强的光动力效应，并通过在CNH上负载Pt NP来增加光热效应，表明这是一种有前途的生物学方法，可用于癌症诊断和癌细胞诊断治疗。
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