Selective Butyrate Esterase Probe for the Rapid Colorimetric and Fluorogenic Identification of Moraxella catarrhalis,Analytical Chemistry

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Selective Butyrate Esterase Probe for the Rapid Colorimetric and Fluorogenic Identification of Moraxella catarrhalis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-11-19 , DOI: 10.1021/acs.analchem.0c03671
Uisung Lee ₁ , Yeon Ho Kim ₂ , Ki Sun Yoon ₂ , Youngmi Kim ₁

Affiliation

Clinical identification of the pathogenic bacterium Moraxella catarrhalis in cultures relies on the detection of bacterial butyrate esterase (C4-esterase) using a coumarin-based fluorogenic substrate, 4-methylumbelliferyl butyrate. However, this classical probe may give false-positive responses because of its poor stability and lack of specificity. Here, we report a new colorimetric and fluorogenic probe design employing a meso-ester-substituted boron dipyrromethene (BODIPY) dye for the specific detection of C4-esterase activity expressed by M. catarrhalis. This new probe has resistance to nonspecific hydrolysis that is far superior to the classical probe and also selectively responds to esterase with rapid colorimetric and fluorescence signal changes and large “turn-on” ratios. The probe was successfully applied to the specific detection of M. catarrhalis with high sensitivity.

中文翻译：

选择性丁酸酯酶探针用于快速比色法和荧光法鉴定卡他莫拉菌

培养物中致病性细菌卡他莫拉菌的临床鉴定依赖于使用基于香豆素的荧光底物4-甲基伞形基丁酸来检测细菌丁酸酯酶（C4-酯酶）。但是，这种经典探针可能会产生假阳性反应，因为其稳定性较差且缺乏特异性。在这里，我们报告了一种新的比色法和荧光探针设计，该探针设计采用了介孔-酯取代的硼二吡咯亚甲基（BODIPY）染料来特异性检测由卡他莫拉氏菌表达的C4-酯酶活性。。这种新型探针对非特异性水解的抵抗力远胜于传统探针，并且可以选择性地响应酯酶，具有快速的比色和荧光信号变化以及大的“开启”率。该探针已成功应用于高灵敏度的粘膜炎莫拉氏菌的特异性检测。

更新日期：2020-12-15

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