Photothermal Microscopy: Imaging the Optical Absorption of Single Nanoparticles and Single Molecules,ACS Nano

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Photothermal Microscopy: Imaging the Optical Absorption of Single Nanoparticles and Single Molecules
ACS Nano ( IF 15.8 ) Pub Date : 2020-11-20 , DOI: 10.1021/acsnano.0c07638
Subhasis Adhikari ₁ , Patrick Spaeth ₁ , Ashish Kar ₂ , Martin Dieter Baaske ₁ , Saumyakanti Khatua ₂ , Michel Orrit ₁

Affiliation

The photothermal (PT) signal arises from slight changes of the index of refraction in a sample due to absorption of a heating light beam. Refractive index changes are measured with a second probing beam, usually of a different color. In the past two decades, this all-optical detection method has reached the sensitivity of single particles and single molecules, which gave birth to original applications in material science and biology. PT microscopy enables shot-noise-limited detection of individual nanoabsorbers among strong scatterers and circumvents many of the limitations of fluorescence-based detection. This review describes the theoretical basis of PT microscopy, the methodological developments that improved its sensitivity toward single-nanoparticle and single-molecule imaging, and a vast number of applications to single-nanoparticle imaging and tracking in material science and in cellular biology.

中文翻译：

光热显微镜：成像单个纳米粒子和单个分子的光吸收。

光热（PT）信号是由于加热光束的吸收导致样品中折射率的轻微变化而产生的。折射率变化是用通常颜色不同的第二根探测光束测量的。在过去的二十年中，这种全光学检测方法已经达到了单个颗粒和单个分子的灵敏度，从而在材料科学和生物学中产生了原始应用。PT显微镜能够对强散射体中的单个纳米吸收体进行散粒噪声限制的检测，并规避了基于荧光检测的许多局限性。这篇综述描述了PT显微镜的理论基础，改进了其对单纳米颗粒和单分子成像灵敏度的方法学发展，

更新日期：2020-12-22

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