当前位置: X-MOL 学术Spectrochim. Acta. A Mol. Biomol. Spectrosc. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A novel dual-function fluorescent probe for the rapid detection of bisulfite and hydrogen peroxide in aqueous solution and living cells
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-11-18 , DOI: 10.1016/j.saa.2020.119226
Ruqiao Zhou , Longxing Niu , Yuefu Hu , Qingrong Qi , Wencai Huang , Li Yang

In this work, Hcy-OB, a novel hemicyanine-based biocompatible dual-function fluorescence probe for bisulfite and H2O2 detection is designed and synthesized. Based on a 1,4-addition reaction, Hcy-OB can be used for bisulfite detection with fast response, high sensitivity and low detection limit (120 nM). In addition, the probe is successfully applied to the detection of bisulfite in aqueous solution. Furthermore, Hcy-OB shows excellent performance for hydrogen peroxide detection with the oxidation of phenylboronic acid. Hcy-OB shows excellent selectivity to H2O2 over other interfering substances with detection limit of H2O2 is calculated to be 70 nM. Most importantly, due to its good cell membrane permeability and low cytotoxicity, Hcy-OB has been applied to monitor and image H2O2 in living cells and mice.



中文翻译:

新型双功能荧光探针,用于快速检测水溶液和活细胞中的亚硫酸氢盐和过氧化氢

在这项工作中,Hcy-OB,新型的基于半菁的生物相容性双功能荧光探针,用于亚硫酸氢盐和H 2 O 2的检测被设计和合成。基于1,4-加成反应,Hcy-OB可用于亚硫酸氢盐检测,具有响应速度快,灵敏度高和检测限低(120 nM)的特点。另外,该探针已成功应用于水溶液中亚硫酸氢盐的检测。此外,Hcy-OB对苯硼酸的氧化具有出色的过氧化氢检测性能。Hcy-OB对H 2 O 2的选择性优于其他干扰物质,检测限为H 2 O 2计算为70 nM。最重要的是,由于Hcy-OB具有良好的细胞膜通透性和低细胞毒性,因此已被用于监测和成像活细胞和小鼠中的H 2 O 2

更新日期:2020-12-07
down
wechat
bug