A highly sensitive quenching molecular imprinting (MIP) photoelectrochemical (PEC) sensor was proposed to detect acrylamide (AM) by using the photoactive composite of ZnO and polypyrrole (PPy) as the PEC signal probe. ZnO, with high electron mobility, excellent chemical and thermal stability as well as good biocompatibility, was selected as the photoelectrically active material. A polypyrrole film was formed on the nanodisk ZnO by electrochemical polymerization, and the recognition site of AM was left on the surface of the PPy film by elution, enabling the specific detection of AM. The transfer of electrons will be hindered when AM is adsorbed on the ZnO/PPy nanocomposites surface, which results in the decrease of photocurrent signal. The proposed molecularly imprinted PEC sensor exhibits significant detection performance of AM in the range of 10⁻¹ M-2.5 × 10⁻⁹ M with a LOD of 2.147 × 10⁻⁹ M (S/N = 3). The use of photoelectrochemical technology combined with molecular imprinting technology enables the PEC sensor to have excellent selectivity, superior repeatability, preferable stability, low cost, and easy construction, providing a new method for the detection of AM. The high recovery rate in the detection of real samples of potato chips and biscuits indicates that the proposed PEC sensor has potential in monitoring the emerging food safety risks.

提出了一种高灵敏度猝灭分子印迹（MIP）光电化学（PEC）传感器，利用ZnO和聚吡咯（PPy）的光活性复合物作为PEC信号探针来检测丙烯酰胺（AM）。 ZnO具有高电子迁移率、优异的化学和热稳定性以及良好的生物相容性，被选为光电活性材料。通过电化学聚合在纳米盘ZnO上形成聚吡咯薄膜，并通过洗脱在PPy薄膜表面留下AM的识别位点，从而实现AM的特异性检测。当AM吸附在ZnO/PPy纳米复合材料表面时，电子的转移会受到阻碍，导致光电流信号减弱。所提出的分子印迹PEC传感器在10 ^-1 M-2.5 × 10 ^-9 M范围内表现出显着的AM检测性能，LOD为2.147 × 10 ^-9 M（S/N = 3）。采用光电化学技术与分子印迹技术相结合，使得PEC传感器具有优异的选择性、优越的重复性、较好的稳定性、低成本、易于施工，为AM的检测提供了一种新的方法。薯片和饼干实际样品检测的高回收率表明所提出的 PEC 传感器在监测新出现的食品安全风险方面具有潜力。