Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport,Angewandte Chemie International Edition

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Bio‐Coreactant‐Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-11-14 , DOI: 10.1002/anie.202012171
Cheng Ma ₁ , Shaojun Wu ₁ , Yang Zhou ₂ , Hui‐Fang Wei ₁ , Jianrong Zhang ₁ , Zixuan Chen ₁ , Jun‐Jie Zhu ₁ , Yuehe Lin ₂ , Wenlei Zhu ₂

Affiliation

A bio‐coreactant‐enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy)₃²⁺ as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a “catalytic route”. Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals. A time‐resolved image sequence discloses the universal edge effect of cellular electroporation due to the influence of the geometric properties of cell membranes on the induced transmembrane voltage. The dynamic transport of Ru(bpy)₃³⁺ in the different cellular compartments unveils the heterogeneous intracellular diffusivity correlating with the actin cytoskeleton. In addition to single‐cell studies, the bio‐coreactant‐enhanced ECL microscopy is used to image a slice of a mouse liver and a colony of Shewanella oneidensis MR‐1.

中文翻译：

生物共反应物增强的电化学发光显微镜下的细胞内结构和运输

生物共活性剂增强的电化学发光（ECL）显微镜可实现细胞内结构和动态转运的ECL成像。该显微镜使用Ru（bpy）₃²⁺作为连接细胞外和细胞内环境的电化学分子天线，并通过“催化途径”使用细胞内生物分子作为ECL反应的共反应物。因此，无需使用多个标记物即可鉴定细胞内结构，并使用核ECL信号检测涉及DNA氧化损伤的自噬。时间分辨图像序列揭示了细胞电穿孔的普遍边缘效应，这是由于细胞膜的几何特性对所感应的跨膜电压的影响。Ru（bpy）₃³⁺的动态输运在不同的细胞区室中揭示了与肌动蛋白细胞骨架相关的异质细胞内扩散率。除单细胞研究外，生物共反应剂增强的ECL显微镜还用于对小鼠肝脏和Shewanella oneidensis MR-1菌落的切片成像。

更新日期：2020-11-14

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