Reduced glutathione (GSH) and the oxidized glutathione (GSSG) are well-known biomolecules in the main constituents of intracellular redox homeostasis system. A rapid, accurate measurement of cellular GSH and GSSG is quite needed in investigating important biochemical events. In this work, we present a novel and sensitive method to monitor intracellular GSH and GSSG concentrations by a portable surface-enhanced Raman spectroscopy (SERS) technique. We introduced a reduction-sensitive reaction-type Raman probe, 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) to initiate GSH reduction, itself concomitantly converts to 2-nitro-5-thiobenzoic acid (TNB) to release a strong SERS signal. In a convenient way of inorganic salt MgSO₄ induced aggregation of silver nanoparticles substrate, we easily implemented a good discrimination between DTNB and TNB, and a quantitative measurement of GSH and GSSG with a high sensitivity of 10 nM. This SERS method proved its feasible applicability in rapidly and sensitively monitoring GSH depletion behaviors of some notorious alkylating agents, i.e., sulfur mustard and nitrogen mustards in ex vitro or in vitro (cellular response). This SERS method may be very worthwhile in cellular detoxication event via the GSH approach and other GSH involved biomedical researches.

还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）是细胞内氧化还原稳态系统主要组成部分中众所周知的生物分子。在调查重要的生化事件时，非常需要快速，准确地测量细胞中的GSH和GSSG。在这项工作中，我们提出了一种新颖而灵敏的方法，通过便携式表面增强拉曼光谱（SERS）技术来监测细胞内GSH和GSSG的浓度。我们引入了对还原反应敏感的拉曼探针5,5'-二硫代双-（2-硝基苯甲酸）（DTNB）来启动GSH还原，它本身也可以转化为2-硝基-5-硫代苯甲酸（TNB）释放强烈的SERS信号。以方便的方式无机盐MgSO ₄诱导银纳米颗粒基质的聚集，我们可以轻松实现DTNB和TNB的良好区分，并以10 nM的高灵敏度定量测定GSH和GSSG。该SERS方法证明了其在快速和灵敏地监测一些臭名昭著的烷基化剂（即硫芥子和氮芥子）在体外或体外的GSH耗竭行为方面的可行性（体外反应）。通过GSH方法和其他GSH涉及的生物医学研究，这种SERS方法在细胞脱毒事件中可能非常值得。