The degradation of an anticancer drug by laccase was investigated for the first time, bringing a new approach to treat these hazardous substances through the direct enzymatic application. Degradations of doxorubicin by laccase were performed in different enzymatic concentrations, pH values and temperatures through kinetic studies. The highest enzymatic degradation of doxorubicin was achieved at pH 7 and 30 ºC, which resembles effluent characteristics from wastewater treatment plants. Assays were carried out in different doxorubicin concentrations to comprehend the enzymatic kinetics of degradation. Michaelis–Menten kinetic parameters obtained were maximum velocity obtained (V_max) of 702.8 µg_DOX·h^-1·L^-1 and Michaelis-Menten constant (K_M) of 4.05 µM, which showed a good affinity for the substrate. The toxicity was evaluated against L-929 cell line, and the degraded doxorubicin solution did not show a reduction in cell viability in the concentration of 250 µg·L^-1. In contrast, the doxorubicin shows a reduction of 27% in cell viability. Furthermore, in the highest tested concentration (1000 µg·L^-1), enzymatic degradation reduced in up 41.4% the toxicity of doxorubicin, which indicates laccase degrades doxorubicin to non-toxic compounds. In conclusion, this study provides a new application to laccase since the results showed great potential to remove anticancer drugs from effluents.

首次研究了漆酶对抗癌药物的降解，为通过直接酶促应用处理这些有害物质带来了新的途径。通过动力学研究在不同的酶浓度、pH 值和温度下进行漆酶对阿霉素的降解。阿霉素的最高酶促降解在 pH 7 和 30 ºC 时实现，这与废水处理厂的出水特征相似。在不同的阿霉素浓度下进行测定以了解降解的酶动力学。获得的 Michaelis-Menten 动力学参数是获得的最大速度 (V _max ) 为 702.8 µg _DOX ·h ^-1 ·L ^-1米氏常数 (K _M ) 为 4.05 µM，对底物表现出良好的亲和力。对L-929细胞系进行毒性评价，降解的多柔比星溶液在250μg·L ^-1的浓度下没有表现出细胞活力的降低。相比之下，多柔比星显示细胞活力降低了 27%。此外，在最高测试浓度（1000 µg·L ^-1）下，酶促降解使阿霉素的毒性降低了41.4%，这表明漆酶将阿霉素降解为无毒化合物。总之，这项研究为漆酶提供了一种新的应用，因为结果显示出从废水中去除抗癌药物的巨大潜力。