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Microfluidic Raman biochip detection of exosomes: a promising tool for prostate cancer diagnosis
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-11-05 , DOI: 10.1039/d0lc00677g Yanlin Wang 1 , Qiaoyu Li 1 , Haimei Shi 2 , Keqi Tang 2 , Liang Qiao 1 , Guopeng Yu 3 , Chuanfan Ding 2 , Shaoning Yu 4
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-11-05 , DOI: 10.1039/d0lc00677g Yanlin Wang 1 , Qiaoyu Li 1 , Haimei Shi 2 , Keqi Tang 2 , Liang Qiao 1 , Guopeng Yu 3 , Chuanfan Ding 2 , Shaoning Yu 4
Affiliation
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Tumor-derived exosomes, which contain RNA, DNA, and proteins, are a potentially rich non-invasive source of biomarkers. However, no efficient isolation or detection methods are yet available. Here, we developed a microfluidic Raman biochip designed to isolate and analyze exosomes in situ. Anti-CD63 magnetic nanoparticles were used to enrich exosomes through mixing channels of a staggered triangular pillar array. EpCAM-functionalized Raman-active polymeric nanomaterials (Raman beads) allow rapid analysis of exosome samples within 1 h, with a quantitative signal at 2230 cm−1. The limit of detection of this biochip approaches 1.6 × 102 particles per mL with 20 μL samples. The newly developed biochip assay was successfully applied in the determination of exosomes from clinical serum samples. Thus, this novel device may have potential as a clinical exosome analysis tool for prostate cancer.
中文翻译：
微流控拉曼生物芯片检测外泌体：前列腺癌诊断的有前途的工具
包含RNA，DNA和蛋白质的肿瘤来源外泌体是生物标记物的潜在丰富非侵入性来源。但是，尚无有效的隔离或检测方法。在这里，我们开发了一种微流控拉曼生物芯片，设计用于原位分离和分析外泌体。抗CD63磁性纳米粒子用于通过交错的三角柱阵列的混合通道富集外泌体。EpCAM官能化的拉曼活性聚合物纳米材料（拉曼珠）可在1小时内快速分析外泌体样品，并在2230 cm -1处产生定量信号。该生物芯片的检测极限接近1.6×10 2每毫升含20μL样品的颗粒。新开发的生物芯片测定法已成功应用于临床血清样品中外泌体的测定。因此，这种新颖的装置可能具有作为前列腺癌临床外泌体分析工具的潜力。
更新日期：2020-11-12
中文翻译：
微流控拉曼生物芯片检测外泌体：前列腺癌诊断的有前途的工具
包含RNA，DNA和蛋白质的肿瘤来源外泌体是生物标记物的潜在丰富非侵入性来源。但是，尚无有效的隔离或检测方法。在这里，我们开发了一种微流控拉曼生物芯片，设计用于原位分离和分析外泌体。抗CD63磁性纳米粒子用于通过交错的三角柱阵列的混合通道富集外泌体。EpCAM官能化的拉曼活性聚合物纳米材料（拉曼珠）可在1小时内快速分析外泌体样品，并在2230 cm -1处产生定量信号。该生物芯片的检测极限接近1.6×10 2每毫升含20μL样品的颗粒。新开发的生物芯片测定法已成功应用于临床血清样品中外泌体的测定。因此，这种新颖的装置可能具有作为前列腺癌临床外泌体分析工具的潜力。
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