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Designable Carboxymethylpachymaran/Metal Ion Architecture on Sunflower Sporopollenin Exine Capsules as Delivery Vehicles for Bioactive Macromolecules
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-11-11 , DOI: 10.1021/acs.jafc.0c05169 Ziyu Deng 1 , Yaqiong Pei 2 , Shishuai Wang 2 , Bin Zhou 3 , Xinyao Hou 1 , Jing Li 1 , Bin Li 1, 4 , Hongshan Liang 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-11-11 , DOI: 10.1021/acs.jafc.0c05169 Ziyu Deng 1 , Yaqiong Pei 2 , Shishuai Wang 2 , Bin Zhou 3 , Xinyao Hou 1 , Jing Li 1 , Bin Li 1, 4 , Hongshan Liang 1
Affiliation
There are multiple obstacles in the gastrointestinal tract (GIT) for oral administration of bioactive macromolecules. Here, we engineered an oral delivery vehicle (sporopollenin exine capsules with carboxymethylpachymaran (CMP)/metal ion modification) with targeted release based on food-grade ingredients and processing operations. Then, the interaction and binding mechanisms between CMP and metal ions in the vehicle were investigated. By using β-galactosidase (β-Gal) as a model protein, the systems were characterized for the surface morphology and monitored by the in vitro release profile of β-Gal. Notably, the CMP/metal ion systems not only markedly decreased the CMP dosage but also achieved a valid long-term release compared with the previously reported CMP system. Among all the systems, the CMP/3% AlCl3 system showed the best ability to control the release with the maximum residual activity of β-Gal at nearly 72% after 24 h of treatment. Subsequently, the interaction mechanism between CMP and metal ions within the system was characterized by the perspectives of microstructure, rheological properties, and spectroscopy characteristics. The results indicated that the low pH conditions are conducive to the further cross-linking of CMP and metal ions, resulting in a high gel strength and thus a dense structure, which can impact the controlled release of β-Gal in the GIT. Overall, the system may be utilized in the administration of medical and functional foods, specifically for the delivery of bioactive proteins via the oral route.
中文翻译:
向日葵孢粉蛋白外来胶囊上可设计的羧甲基pachymaran /金属离子结构作为生物活性大分子的载体
胃肠道(GIT)口服生物活性大分子存在多种障碍。在这里,我们设计了基于食品级成分和加工操作的靶向释放的口服给药载体(具有羧甲基蝶草烷(CMP)/金属离子修饰的孢粉花青素胶囊)。然后,研究了车辆中CMP与金属离子之间的相互作用和结合机理。通过使用β-半乳糖苷酶(β-Gal)作为模型蛋白,对系统的表面形貌进行表征,并通过β-Gal的体外释放曲线进行监测。值得注意的是,与先前报道的CMP系统相比,CMP /金属离子系统不仅显着降低了CMP剂量,而且实现了有效的长期释放。在所有系统中,CMP / 3%AlCl3个系统在处理24小时后表现出最佳的释放控制能力,β-Gal的最大残留活性接近72%。随后,通过微观结构,流变性质和光谱学特性的角度对系统中CMP与金属离子之间的相互作用机理进行了表征。结果表明,低pH条件有利于CMP与金属离子的进一步交联,从而导致较高的凝胶强度,从而形成致密的结构,从而影响GIT中β-Gal的受控释放。总体而言,该系统可用于医疗和功能性食品的给药,特别是用于通过口服途径递送生物活性蛋白。
更新日期:2020-11-25
中文翻译:
向日葵孢粉蛋白外来胶囊上可设计的羧甲基pachymaran /金属离子结构作为生物活性大分子的载体
胃肠道(GIT)口服生物活性大分子存在多种障碍。在这里,我们设计了基于食品级成分和加工操作的靶向释放的口服给药载体(具有羧甲基蝶草烷(CMP)/金属离子修饰的孢粉花青素胶囊)。然后,研究了车辆中CMP与金属离子之间的相互作用和结合机理。通过使用β-半乳糖苷酶(β-Gal)作为模型蛋白,对系统的表面形貌进行表征,并通过β-Gal的体外释放曲线进行监测。值得注意的是,与先前报道的CMP系统相比,CMP /金属离子系统不仅显着降低了CMP剂量,而且实现了有效的长期释放。在所有系统中,CMP / 3%AlCl3个系统在处理24小时后表现出最佳的释放控制能力,β-Gal的最大残留活性接近72%。随后,通过微观结构,流变性质和光谱学特性的角度对系统中CMP与金属离子之间的相互作用机理进行了表征。结果表明,低pH条件有利于CMP与金属离子的进一步交联,从而导致较高的凝胶强度,从而形成致密的结构,从而影响GIT中β-Gal的受控释放。总体而言,该系统可用于医疗和功能性食品的给药,特别是用于通过口服途径递送生物活性蛋白。