Bufalin is an anticancer drug extract from traditional Chinese medicine. Several articles about bufalin have been published. However, the literature on bufalin has not yet been systematically studied. This study aimed to identify the study status and knowledge structures of bufalin and to summarize the antitumor mechanism. Data were retrieved and downloaded from the PubMed database. The softwares of BICOMB, gCLUTO, Ucinet 6.0, and NetDraw2.084 were used to analyze these publications. The bufalin related genes were recognized and tagged by ABNER software. Then these BF-related genes were performed by Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis, and protein-protein interaction (PPI) network analysis. A total of 474 papers met the search criteria from 2000 to 2019. By biclustering clustering analysis, the 50 high-frequency main MeSH terms/subheadings were classified into 5 clusters. The clusters of drug therapy and the mechanism of bufalin were hotspot topics. A total of 50 genes were identified as BF-related genes. PPI network analysis showed that inducing apoptosis was the main effect of bufalin, and apoptosis-related gene Caspase 3 was the most reported by people. Bufalin could inhibit the proliferation, invasion, and metastasis of cancer cells through multiple signaling pathways, such as PI3K/AKT, Hedgehog, MAPK/JNK, Wnt/[Formula: see text]-catenin, TGF-[Formula: see text]/Smad, Integrin signaling pathway, and NF-KB signaling pathway via KEGG analysis. Through the quantitative analysis of bufalin literature, we revealed the research status and hot spots in this field and provided some guidance for further research.

中文翻译：

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Bufalin：文本挖掘和生物信息学对研究热点和抗肿瘤机制的系统评价

蟾蜍灵是一种从中药中提取的抗癌药物。已经发表了几篇关于蟾蜍灵的文章。然而，关于蟾蜍灵的文献尚未得到系统研究。本研究旨在确定蟾毒灵的研究现状和知识结构，并总结其抗肿瘤机制。从 PubMed 数据库中检索和下载数据。使用 BICOMB、gCLUTO、Ucinet 6.0 和 NetDraw2.084 软件对这些出版物进行分析。ABNER软件识别并标记蟾毒灵相关基因。然后通过基因本体论（GO）富集分析、京都基因和基因组百科全书（KEGG）通路分析和蛋白质-蛋白质相互作用（PPI）网络分析对这些BF相关基因进行分析。从 2000 年到 2019 年，共有 474 篇论文符合检索条件。通过双聚类聚类分析，将 50 个高频主要 MeSH 术语/子标题分为 5 个聚类。药物治疗的集群和蟾蜍灵的作用机制是热点话题。共有50个基因被鉴定为BF相关基因。PPI网络分析显示，蟾毒灵主要作用是诱导细胞凋亡，其中凋亡相关基因Caspase 3被人们报道最多。蟾毒灵可通过PI3K/AKT、Hedgehog、MAPK/JNK、Wnt/[分子式：见正文]-catenin、TGF-[分子式：见正文]等多种信号通路抑制癌细胞的增殖、侵袭和转移。通过 KEGG 分析的 Smad、整合素信号通路和 NF-KB 信号通路。通过对蟾蜍灵文献的定量分析，