A High‐Throughput Screening Method for the Directed Evolution of Hydroxynitrile Lyase towards Cyanohydrin Synthesis,ChemBioChem

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A High‐Throughput Screening Method for the Directed Evolution of Hydroxynitrile Lyase towards Cyanohydrin Synthesis
ChemBioChem ( IF 2.6 ) Pub Date : 2020-11-04 , DOI: 10.1002/cbic.202000658
Yu-Cong Zheng ₁ , Liang-Yi Ding ₁ , Qiao Jia ₁ , Zuming Lin ₂ , Ran Hong _{2,

3} , Hui-Lei Yu ₁ , Jian-He Xu ₁

Affiliation

Making biohydrocyanation measurable: Hydroxynitrile lyases (HNLs) catalyze the enantioselective cleavage/formation of cyanohydrins. However, current methods for determining hydrocyanation are not suitable for mass screening of mutants for protein engineering of these biocatalysts. We demonstrate herein a chromogenic high‐throughput screening method for cyanohydrin synthesis that is validated by either substrate profiling or the directed evolution of HNLs.

中文翻译：

一种用于将羟基腈裂解酶定向进化为氰醇合成的高通量筛选方法

使生物氢氰化可测量：羟腈裂解酶 (HNL) 催化对映选择性裂解/形成氰醇。然而，目前确定氢氰化的方法不适合对这些生物催化剂的蛋白质工程进行突变体的大规模筛选。我们在此展示了一种用于氰醇合成的显色高通量筛选方法，该方法通过底物分析或 HNL 的定向进化进行验证。

更新日期：2020-11-04

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