It is very important to exploit real-time, ultrasensitive and specific visualization detection methods for hypochlorous acid/hypochlorite (HOCl/ClO^–) in biological systems as they are the guardians of the human immune system against pathogens invasion. In our work, we designed a novel reversible naphthalimide-based fluorescent probe NAP-OH to recognize HClO/ClO^– with a unique selective colorimetric and fluorescent response, a short response time (<8 s) and a high sensitivity (10.3 nM). In addition, NAP-OH exhibits a novel on-off-on fluorescence response to ClO^–/ascorbic acid (AA) with good cycle stability. The fluorescence signal is quenched because HClO/ClO^– oxidizes the subunit of NAP-OH to the segment 2,2,6,6-tetramethyl-1-oxo-piperidinium in NAP-O, which can be reduced by AA with the recovery of fluorescence. Finally, the confocal fluorescence imaging has been performed, which proves that NAP-OH can satisfactorily monitor intracellular endogenous and exogenous HClO/AA redox cycles.

它是利用实时的，为次氯酸/次氯酸（次氯酸/ CLO超灵敏和具体的可视化检测方法非常重要^- ）在生物系统中，因为它们是抵御病原体侵入人体免疫系统的监护人。在我们的工作中，我们设计了一种新的基于萘二甲酰亚胺可逆的荧光探针NAP-OH识别的HClO / CLO ^-具有独特的选择性比色和荧光响应，短的响应时间（<8秒）和高灵敏度（10.3纳米）。此外，NAP-OH显示出新颖的开-关-荧光响应至C10 ^- /抗坏血酸（AA）具有良好的循环稳定性。荧光信号被猝灭，因为HClO / ClO ^–氧化的亚基NAP-OH到段2,2,6,6-四甲基-1-氧代-哌啶在NAP-O ，其可通过AA减小与荧光的恢复。最后，进行了共聚焦荧光成像，证明NAP-OH可以令人满意地监测细胞内源性和外源性HClO / AA氧化还原循环。