Spatial and Temporal Dynamics in Attached and Suspended Bacterial Communities in Three Drinking Water Distribution Systems with Variable Biological Stability,Environmental Science & Technology

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Spatial and Temporal Dynamics in Attached and Suspended Bacterial Communities in Three Drinking Water Distribution Systems with Variable Biological Stability
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-11-02 , DOI: 10.1021/acs.est.0c04532
Charlotte D. Vavourakis ₁ , Leo Heijnen ₁ , Marjolein C. F. M. Peters ₂ , Leonie Marang ₂ , Henk A. M. Ketelaars ₂ , Wim A. M. Hijnen ₂

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Microbial presence and regrowth in drinking water distribution systems (DWDSs) is routinely monitored to assess the biological stability of drinking water without a residual disinfectant, but the conventional microbiological culture methods currently used target only a very small fraction of the complete DWDS microbiome. Here, we sequenced 16S rRNA gene amplicons to elucidate the attached and suspended prokaryotic community dynamics within three nonchlorinated DWDSs with variable regrowth conditions distributing similarly treated surface water from the same source. One rural location, with less regrowth related issues, differed most strikingly from the other two urban locations by the exclusive presence of Pseudonocardia (Actinobacteria) in the biofilm and the absence of Limnobacter (Betaproteobacteriales) in the water and loose deposits during summer. There was a dominant seasonal effect on the drinking water microbiomes at all three locations. For one urban location, it was established that the most significant changes in the microbial community composition on a spatial scale occurred shortly after freshly treated water entered the DWDS. However, summerly regrowth of Limnobacter, one of the dominant genera in the distributed drinking water, already occurred in the clean water reservoir at the treatment plant before further distribution. The highlighted bacterial lineages within these highly diverse DWDS communities might be important new indicators for undesirable regrowth conditions affecting the final drinking water quality.

中文翻译：

具有可变生物稳定性的三种饮用水分配系统中细菌附着和悬浮细菌的时空动态

常规监测饮用水分配系统（DWDS）中的微生物存在和再生，以评估没有残留消毒剂的饮用水的生物稳定性，但是当前使用的常规微生物培养方法仅针对完整DWDS微生物组的一小部分。在这里，我们对16S rRNA基因扩增子进行了测序，以阐明三个非氯化DWDS内附着和悬浮的原核生物群落动态，这些非DWDS具有可变的再生条件，并从同一水源分配经过相似处理的地表水。一个农村地区，与再生长相关的问题较少，与其他两个城市地区的差异最为明显，因为生物膜中仅存在假性心动菌（放线菌），而没有生物膜。水中有Limnobacter（Betaproteobacteriales），夏季沉积物稀疏。在所有三个地点，饮用水微生物群落都有明显的季节性影响。对于一个城市地区，已经确定，在新鲜处理的水进入DWDS之后不久，微生物群落组成在空间尺度上发生了最显着的变化。但是，Limnobacter在分配饮用水中占主导地位的属之一在夏季再生长，在进一步分配之前，已经在处理厂的净水水库中发生过。在这些高度多样化的DWDS社区中，突出显示的细菌谱系可能是重要的新指标，可以说明影响最终饮用水质量的不良再生条件。

更新日期：2020-11-17

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