A 180-day incubation study was conducted to evaluate the effects of risk elements (REs) on organic carbon use and microbial activities in organic soils in the Arctic during the summer snowmelt period. Soils were artificially spiked with Cd, Pb, Cr, Ni, Cu, As, and a combination of these REs according to the levels measured in Arctic soils from REs-polluted industrial sites. During the incubation period, microbial activities and microbial biomass carbon (MBC) formation were inhibited, and microbial quotient (qCO2) values were relatively high in the spiked soils indicating that more energy was used by microbes for maintenance under REs stress. Meanwhile, microbial metabolism was significantly restrained. Microbial Specific phospholipid fatty acids (PLFAs) were reduced in RE spiked soils relative to the control, especially in the As- and multi-RE-spiked soils. The abundance of both fungi and bacteria was reduced in response to RE amendments by 14–24% and 1–55%, respectively. PLFA biomarkers indicated a shift in soil microbial community structure and activities influenced by REs, consequently having a negative effect on soil organic carbon degradation. This study addresses the knowledge gap regarding the alternation of biochemical reactions in Arctic soils under anthropogenic REs with relevant contamination levels.

进行了一项为期180天的孵化研究，以评估夏季融雪期中危险因素（RE）对北极有机土壤中有机碳利用和微生物活动的影响。根据在受到REs污染的工业区的北极土壤中测得的水平，将Cd，Pb，Cr，Ni，Cu，As以及这些RE的混合物人工掺入土壤。在潜伏期，微生物活性和微生物生物量碳（MBC）的形成受到抑制，微生物商（q加标土壤中的CO2值相对较高，表明微生物在RE胁迫下将更多的能量用于维护。同时，微生物代谢被显着抑制。相对于对照，RE加标土壤中的微生物特异性磷脂脂肪酸（PLFA）降低​​，尤其是As和多RE加标土壤中的微生物。响应RE修正，真菌和细菌的丰度分别降低了14-24％和1-55％。PLFA生物标志物表明土壤微生物群落结构和活性受到可再生能源的影响，因此对土壤有机碳降解产生负面影响。这项研究解决了在具有相关污染水平的人为稀土环境下，北极土壤中生化反应交替的知识缺口。