Bacterial pathogen detection by conventional culture‐based and recent alternative (polymerase chain reaction, isothermal amplification, enzyme linked immunosorbent assay, bacteriophage amplification, and gold nanoparticle aggregation) methods in food samples: A review,Journal of Food Safety

当前位置： X-MOL 学术 › J. Food Saf. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Bacterial pathogen detection by conventional culture‐based and recent alternative (polymerase chain reaction, isothermal amplification, enzyme linked immunosorbent assay, bacteriophage amplification, and gold nanoparticle aggregation) methods in food samples: A review
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-10-27 , DOI: 10.1111/jfs.12870
Sang‐Oh Kim ₁ , Sang‐Soon Kim ₂

Affiliation

The rapid, sensitive, and selective detection of foodborne pathogens is important to ensure food safety. Culture medium‐based methods for bacteria detection have long been used since Robert Koch's first finding. These methods are simple and cheap but have limitations, such as being time‐consuming, labor‐intensive, and having low selectivity. In this regard, several alternative detection methods have been reported. Among these, recent studies related to the application of polymerase chain reaction, isothermal amplification, bacteriophage amplification, enzyme‐linked immunosorbent assay, and gold nanoparticle aggregation for detection of pathogens in food are discussed in this review. The principles, advantages, and disadvantages of alternative methods are covered, including their rapidity, sensitivity, and selectivity. Finally, regulations related to bacterial pathogen detection in the United States and South Korea were compared with remarks for their progress.

中文翻译：

食品样品中基于常规培养物和最新替代方法（聚合酶链反应，等温扩增，酶联免疫吸附测定，噬菌体扩增和金纳米颗粒聚集）的细菌病原体检测：综述

对食源性病原体的快速，灵敏和选择性检测对于确保食品安全至关重要。自从罗伯特·科赫（Robert Koch）首次发现以来，就一直使用基于培养基的细菌检测方法。这些方法既简单又便宜，但有局限性，例如费时，费力，选择性低。在这方面，已经报道了几种替代的检测方法。其中，本文综述了有关聚合酶链反应，等温扩增，噬菌体扩增，酶联免疫吸附测定和金纳米颗粒聚集体在食品中病原体检测中的应用的最新研究。涵盖了替代方法的原理，优点和缺点，包括它们的快速性，敏感性和选择性。最后，

更新日期：2020-10-27

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南