Carbohydrate Polymers ( IF 10.7 ) Pub Date : 2020-10-22 , DOI: 10.1016/j.carbpol.2020.117287
Peiqian Zhang , Rongxin Su , Yuhao Duan , Mei Cui , Renliang Huang , Wei Qi , Zhimin He , Wim Thielemans
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Effective binding between cellulases and cellulose is essential for enzymatic hydrolysis of lignocellulose. Expansin can loosen the cellulose structure and can enhance the efficiency of cellulase. However, possible synergy between cellulases and expansin is not clear. In this work, the real-time adsorption of exoglucanases (Cel7A) or endoglucanases (Cel7B) with Bacillus subtilis expansin (BsEXLX1) and the enzymatic hydrolysis of cellulose were followed using quartz crystal microbalance with dissipation (QCM-D). Initial adsorption rate, adsorption capacity, and pseudo-steady-state rate of cellulose hydrolysis by Cel7A/Cel7B increased in the presence of BsEXLX1. When injecting Cel7A or Cel7B together with BsEXLX1 at a mass ratio of 1:1, the hydrolysis rate was almost 5 times the rate for Cel7A or Cel7B alone at 25 °C. These results increase our understanding of the real-time synergism between cellulases and expansin on cellulose, as well as the impact of their synergy on the enzymatic hydrolysis of cellulose.
中文翻译:
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内切/外切葡聚糖酶和扩展酶之间的协同作用增强了酶的吸附和纤维素的转化
纤维素酶和纤维素之间的有效结合对于木质纤维素的酶促水解至关重要。扩展酶可以使纤维素结构疏松并可以提高纤维素酶的效率。但是,纤维素酶和弹性蛋白之间可能的协同作用尚不清楚。在这项工作中,利用枯草芽孢杆菌扩展酶(Bs EXLX1)实时吸附外切葡聚糖酶(Cel7A)或内切葡聚糖酶(Cel7B),并使用带耗散的石英晶体微天平(QCM-D)进行纤维素的酶水解。在Bs EXLX1的存在下,纤维素被Cel7A / Cel7B水解的初始吸附速率,吸附容量和拟稳态速率增加。与B一起注入Cel7A或Cel7B时EXLX1的质量比为1:1,水解速率几乎是25°C下Cel7A或Cel7B单独水解速率的5倍。这些结果增加了我们对纤维素酶和弹性蛋白在纤维素上的实时协同作用以及它们的协同作用对纤维素酶水解的影响的理解。