Traditional Anterior Cruciate Ligament (ACL) reconstruction is commonly performed using an allograft or autograft and possesses limitations such as donor site morbidity, decreased range of motion, and potential infection. However, a biodegradable synthetic graft could greatly assist in the prevention of such restrictions after ACL reconstruction. In this study, artificial grafts were generated using “wet” and “dry” electrospinning processes with a biodegradable elastomer, poly (ester urethane) urea (PEUU), and were evaluated in vitro and in vivo in a rat model. Four groups were established: (1) Wet PEUU artificial ligament, (2) Dry PEUU artificial ligament, (3) Dry polycaprolactone artificial ligament (PCL), and (4) autologous flexor digitorum longus tendon graft. Eight weeks after surgery, the in vivo tensile strength of wet PEUU ligaments had significantly increased compared to the other synthetic ligaments. These results aligned with increased infiltration of host cells and decreased inflammation within the wet PEUU grafts. In contrast, very little cellular infiltration was observed in PCL and dry PEUU grafts. Micro-computed tomography analysis performed at 4 and 8 weeks postoperatively revealed significantly smaller bone tunnels in the tendon autograft and wet PEUU groups. The Wet PEUU grafts served as an adequate functioning material and allowed for the creation of tissues that closely resembled the ACL.

传统的前交叉韧带（ACL）重建通常使用同种异体移植或自体移植进行，并具有局限性，例如供体部位发病率，活动范围减少和潜在感染。但是，可生物降解的合成移植物可以极大地帮助预防ACL重建后的此类限制。在这项研究中，使用“湿”和“干式”静电纺纱方法中与可生物降解的弹性体，聚（酯-氨基甲酸酯）脲（PEUU）产生人工移植物，并评价在体外和体内在大鼠模型中。建立四组：（1）湿PEUU人工韧带，（2）干PEUU人工韧带，（3）干聚己内酯人工韧带（PCL），和（4）自体屈指长肌腱移植。手术八周后，在体内与其他合成韧带相比，湿PEUU韧带的拉伸强度显着提高。这些结果与增加的宿主细胞浸润和减少的湿PEUU移植物中的炎症一致。相反，在PCL和干PEUU移植物中观察到的细胞浸润很少。术后4周和8周进行的微计算机断层扫描分析显示，自体肌腱和湿PEUU组的骨隧道明显较小。湿PEUU移植物可作为适当的功能材料，并允许创建与ACL非常相似的组织。