Nature Chemistry ( IF 19.2 ) Pub Date : 2020-10-19 , DOI: 10.1038/s41557-020-00554-5
Emily D Cosco 1, 2 , Anthony L Spearman 1 , Shyam Ramakrishnan 2 , Jakob G P Lingg 2 , Mara Saccomano 2 , Monica Pengshung 1 , Bernardo A Arús 2 , Kelly C Y Wong 1 , Sarah Glasl 3 , Vasilis Ntziachristos 3 , Martin Warmer 2 , Ryan R McLaughlin 1 , Oliver T Bruns 2 , Ellen M Sletten 1
|
High-resolution, multiplexed experiments are a staple in cellular imaging. Analogous experiments in animals are challenging, however, due to substantial scattering and autofluorescence in tissue at visible (350–700 nm) and near-infrared (700–1,000 nm) wavelengths. Here, we enable real-time, non-invasive multicolour imaging experiments in animals through the design of optical contrast agents for the shortwave infrared (SWIR, 1,000–2,000 nm) region and complementary advances in imaging technologies. We developed tunable, SWIR-emissive flavylium polymethine dyes and established relationships between structure and photophysical properties for this class of bright SWIR contrast agents. In parallel, we designed an imaging system with variable near-infrared/SWIR excitation and single-channel detection, facilitating video-rate multicolour SWIR imaging for optically guided surgery and imaging of awake and moving mice with multiplexed detection. Optimized dyes matched to 980 nm and 1,064 nm lasers, combined with the clinically approved indocyanine green, enabled real-time, three-colour imaging with high temporal and spatial resolutions.
中文翻译:

短波红外聚次甲基荧光团与激发激光器匹配,可实现非侵入性、多色实时体内成像
高分辨率、多重实验是细胞成像的主要内容。然而,由于组织在可见光(350-700 nm)和近红外(700-1,000 nm)波长下存在大量散射和自发荧光,因此在动物身上进行类似实验具有挑战性。在这里,我们通过设计短波红外(SWIR,1,000–2,000 nm)区域的光学造影剂和成像技术的互补进步,在动物身上进行实时、非侵入性多色成像实验。我们开发了可调谐的短波红外发射黄鎓聚次甲基染料,并建立了此类明亮短波红外造影剂的结构和光物理性质之间的关系。与此同时,我们设计了一种具有可变近红外/短波红外激发和单通道检测的成像系统,有助于光学引导手术的视频速率多色短波红外成像,以及通过多重检测对清醒和运动的小鼠进行成像。与 980 nm 和 1,064 nm 激光匹配的优化染料,与临床批准的吲哚菁绿相结合,实现了具有高时间和空间分辨率的实时三色成像。