绿色海藻 Valoniopsis pachynema 的酸水解优化和混合营养微藻生物量和脂质生产的方法,Renewable Energy

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绿色海藻 Valoniopsis pachynema 的酸水解优化和混合营养微藻生物量和脂质生产的方法
Renewable Energy ( IF 9.0 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.renene.2020.10.062
M. Lakshmikandan , A.G. Murugesan , Shuang Wang , Abd El-Fatah Abomohra

摘要采用响应面法(RSM)，采用稀硫酸水解优化绿海藻Valoniopsis pachynema 的糖化过程，并对回收的水解产物进行表征。酸水解产物总糖回收率的 RSM 定量评估表明，3% 的稀硫酸足以在 60 °C 下培养 2.15 小时，以恢复最佳糖化水平。海藻的傅里叶变换红外光谱 (FTIR) 和扫描电子显微镜 (SEM) 与能量色散 X 射线分析 (EDAX) 图像证明海藻表面多糖被 3% 稀硫酸水解，进一步这种处理显示聚合物溶解度提高与其他浓度相比。酸处理的海藻颗粒在微藻 C. vulgaris MSU-AGM 14 的粘附分析中表明有效定植并促进生长。通过与藻类培养基和不同浓度的酸水解物结合显示出更高的生物量（0.111 ± 0.001 g L-1 d-） 1) 和脂质生产力 (18.18 ± 0.19 mg L-1 d-1) 和改善混合营养条件下的元素含量。这些结果证明，混合营养培养通过细胞外多糖的存在增强了微藻的生长和脂质生产力，并导致初始定植和延长的生长时间。总体结果表明，稀释的 3% 硫酸水解从绿海藻 V. pachynema 中回收了大量的总糖含量 (38.5 ± 0.2 mg g-1)，并显着促进了微藻生物量和脂质生产力。

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Optimization of acid hydrolysis on the green seaweed Valoniopsis pachynema and approach towards mixotrophic microalgal biomass and lipid production

Abstract The dilute sulfuric acid hydrolysis was employed to optimize saccharification of the green seaweed Valoniopsis pachynema by response surface methodology (RSM) and the retrieved hydrolysates were characterized. The RSM quantitative evaluation of total sugar recovery on acid hydrolysates indicated that 3% dilute sulfuric acid was sufficient to retrieve optimum level of saccharification with 2.15 h incubation time at 60 °C. Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive X-Ray Analysis (EDAX) images of seaweed evidenced that seaweed surface polysaccharides were hydrolyzed by 3% dilute sulfuric acid, further this treatment showed improved polymer dissolution when compared to other concentrations. The acid treated seaweed pellets indicated effective colonization and encouraged growth in adhesion analysis of microalgae C. vulgaris MSU-AGM 14. By combining with algae culture medium and at different concentrations of acid hydrolysate showed higher biomass (0.111 ± 0.001 g L−1 d−1) and lipid productivity (18.18 ± 0.19 mg L−1 d−1) and improved elemental contents in mixotrophic condition. These results evidenced that mixotrophic cultivation enhances microalgae growth and lipid productivity by presence of extracellular polysaccharide and leads to initial colonization and prolonged period of growth. The overall results expressed that dilute 3% sulfuric acid hydrolysis retrieved high amount of total sugar content (38.5 ± 0.2 mg g−1) from green seaweed V. pachynema and promotes microalgae biomass and lipid productivity significantly.

更新日期：2021-02-01

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