Engineering and Application of a Myoglobin Binding Split Aptamer,Analytical Chemistry

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Engineering and Application of a Myoglobin Binding Split Aptamer
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-10-14 , DOI: 10.1021/acs.analchem.0c02869
Shaoyuan Li ₁ , Yan Zheng ₁ , Qingqing Zou ₁ , Guofu Liao ₁ , Xiaofeng Liu ₁ , Liyuan Zou ₁ , Xiaohai Yang ₁ , Qing Wang ₁ , Kemin Wang ₁

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Given that a split aptamer provides a chance for the development of a sandwich assay for targets with only one aptamer, it has received extensive attention in biosensing. However, due to the lack of binding mechanisms and reliable methods, there were still a few split aptamers that bind to proteins. In this work, cardiac biomarker myoglobin (Myo) was selected as a model, a new strategy of engineering split aptamers was explored with atomic force spectroscopy (AFM), and split aptamers against target protein could be achieved by choosing the optimal binding probability between split aptamers and target. Then, the obtained split aptamers were designed for Myo detection based on dynamic light scattering (DLS). The results demonstrated that the obtained split aptamers could be used to detect targets in human serum. The strategy of engineering split aptamers has the advantages of being intuitive and reliable and could be a general strategy for obtaining split aptamers.

中文翻译：

肌红蛋白结合分裂适体的工程和应用

鉴于分裂的适体为仅使用一种适体的靶标进行夹心测定提供了机会，因此在生物传感领域受到了广泛关注。然而，由于缺乏结合机制和可靠的方法，仍然有一些与蛋白质结合的分裂适体。在这项工作中，选择了心脏生物标记物肌红蛋白（Myo）作为模型，通过原子力光谱（AFM）探索了一种工程化分裂适体的新策略，并且可以通过选择分裂之间的最佳结合概率来实现针对靶蛋白的分裂适体。适体和目标。然后，将获得的分裂适体设计用于基于动态光散射（DLS）的Myo检测。结果表明，所得分裂适体可用于检测人血清中的靶标。

更新日期：2020-11-03

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