This work introduces photography-based photometry, as complementary to digital image colorimetry, by demonstrating that historical photography can be repurposed to enable UV photometric analysis, using inexpensive and ubiquitous materials. Analysis is performed by illuminating the samples with UV radiation and then capturing the transmitted irradiation on the surface of a photographic paper through photochemical reduction of its photosensitive coating. A visible image is then formed on the paper surface and is recorded as a digital photograph. The color intensity of the image is directly proportional to the concentration of the analytes in the sample and is quantified in the RGB color system. By selecting the appropriate light source and photosensitive paper, sensitivity to light can be selected to approximate the absorbance spectra of the target analyte thus accomplishing good selectivity and high sensitivity. On the basis of this principle, a variety of assays were used to demonstrate that photography-based photometry, can be used to perform accurate UV absorbance measurements over a wide spectral range, in a high throughput format (i.e. microtiter plates) and at low cost as compared to expensive microplate spectrophotometers. Depending on the photosensitivity of the photographic paper, linear ranges and detection limits that are similar or lower up to an order of magnitude to those obtained with standard spectrophotometry were achieved. Importantly, a single device configuration can be used for all assays, irrespectively of the maximum absorbance wavelength, which significantly simplifies its operation thus holding great promise for routine applications in resource-limited settings even by non-experts.

这项工作通过证明历史摄影可以重新利用廉价的无处不在的材料进行UV光度分析，从而引入了基于摄影的光度法，作为对数字图像色度的补充。通过用紫外线辐射照亮样品，然后通过光敏还原其感光涂层，将透射的辐射捕获在相纸表面上来进行分析。然后在纸张表面上形成可见图像，并将其记录为数码照片。图像的颜色强度与样品中分析物的浓度成正比，并在RGB颜色系统中进行定量。通过选择合适的光源和光敏纸，可以选择对光的灵敏度以接近目标分析物的吸收光谱，从而实现良好的选择性和高灵敏度。基于此原理，使用了多种测定方法来证明基于摄影的测光法可用于在宽光谱范围内以高通量格式（即微量滴定板）以低成本进行准确的紫外线吸收率测量。与昂贵的微孔板分光光度计相比。取决于相纸的光敏性，获得了与标准分光光度法所获得的线性范围和检测极限相近或更低的数量级。重要的是，单个仪器配置可用于所有测定，而与最大吸收波长无关，