Tunable plasmon-assisted electrochemiluminescence strategy for determination of the rapidly accelerated fibrosarcoma B-type (BRAF) gene using concave gold nanocubes,Microchimica Acta

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Tunable plasmon-assisted electrochemiluminescence strategy for determination of the rapidly accelerated fibrosarcoma B-type (BRAF) gene using concave gold nanocubes
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-10-09 , DOI: 10.1007/s00604-020-04584-1
Qian Zhang _{1,

2} , Zihui Liang ₁ , Yixin Nie ₁ , Xin Zhang ₁ , Qiang Ma ₁

Affiliation

A tunable plasmon-assisted electrochemiluminescence (ECL) strategy is reported using concave Au nanocubes (Au CBs) for rapidly accelerated fibrosarcoma B-type (BRAF) detection. Concave Au CBs exhibit a strong surface plasmon coupling (SPC) effect between its sharp apexes and edges. The high spectral overlap with graphite phase carbon nitride quantum dots (g-C3N4 QDs) is achieved by tuning surface plasmon absorption peak of the concave Au CBs. It maximizes the SPC effect and enhances the ECL signal of g-C3N4 QDs 3-fold. The SPC effect of Au CBs is twice as high as with Au NPs. We also employed a toehold-mediated strand displacement (TMSD) strategy for sensitive target recycling amplification. Under optimal conditions, this sensor can determine BRAF gene from 1 pM to 1 nM with a detection limit of 3.06 × 10−5 nM (S/N = 3) and RSD 3.67%. With the aid of the TMSD strategy and tunable plasmon-assisted ECL sensing mode, this sensor also exhibits good analytical performance in human serum with satisfactory recovery of 90.2~109%. The proposed strategy provides a promising method to effectively enhance spectral overlap and detect BRAF gene.

中文翻译：

使用凹面金纳米立方体测定快速加速纤维肉瘤 B 型 (BRAF) 基因的可调谐等离子体辅助电化学发光策略

据报道，一种可调谐等离子体辅助电化学发光 (ECL) 策略使用凹形金纳米立方体 (Au CBs) 用于快速加速纤维肉瘤 B 型 (BRAF) 检测。凹面 Au CB 在其尖锐的顶点和边缘之间表现出强烈的表面等离子体耦合 (SPC) 效应。与石墨相氮化碳量子点 (g-C3N4 QDs) 的高光谱重叠是通过调整凹面 Au CBs 的表面等离子体吸收峰来实现的。它最大限度地提高了 SPC 效应并将 g-C3N4 QD 的 ECL 信号增强了 3 倍。Au CBs 的 SPC 效应是 Au NPs 的两倍。我们还采用立足点介导的链置换 (TMSD) 策略进行敏感目标循环放大。在最佳条件下，该传感器可以检测 1 pM 至 1 nM 的 BRAF 基因，检测限为 3.06 × 10−5 nM (S/N = 3)，RSD 为 3.67%。借助 TMSD 策略和可调等离子辅助 ECL 传感模式，该传感器在人血清中也表现出良好的分析性能，回收率为 90.2~109%。所提出的策略为有效增强光谱重叠和检测 BRAF 基因提供了一种有前途的方法。

更新日期：2020-10-09

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