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Aptamer Generated by Cell-SELEX for Specific Targeting of Human Glioma Cells
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-10-08 , DOI: 10.1021/acsami.0c11878 Ningqin Lin 1 , Liang Wu 1 , Xing Xu 2 , Qiaoyi Wu 1 , Yuzhe Wang 1 , Haicong Shen 2 , Yanling Song 2 , Hongyao Wang 1 , Zhi Zhu 2 , Dezhi Kang 1 , Chaoyong Yang 2, 3
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-10-08 , DOI: 10.1021/acsami.0c11878 Ningqin Lin 1 , Liang Wu 1 , Xing Xu 2 , Qiaoyi Wu 1 , Yuzhe Wang 1 , Haicong Shen 2 , Yanling Song 2 , Hongyao Wang 1 , Zhi Zhu 2 , Dezhi Kang 1 , Chaoyong Yang 2, 3
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The most prevalent primary brain tumors are gliomas, which start in the glial cells. Although there have been significant technological advances in surgery and radio-chemotherapy, the prognosis and survival of patients with malignant gliomas remain poor. For routine diagnosis of glioma, computed tomography and magnetic resonance imaging primarily depend on anatomical changes and fail to detect the cellular changes that occur early in the development of malignant gliomas. Therefore, it is urgent to find effective molecular diagnostic tools to detect early stages of malignant gliomas. Currently, cell-based Systematic Evolution of Ligands by EXponential enrichment (cell-SELEX) technology is one effective tool to obtain DNA or RNA aptamers capable of differentiating the molecular signatures among different types of cell lines. Using cell-SELEX, we generated and characterized an aptamer, termed S6-1b, that can distinguish the molecular differences between glioma cell line SHG44 and human astrocytes. Under the conditions of 4 and 37 °C, respectively, the dissociation constants of aptamer–cell interaction were both measured in the low nanomolar range. The aptamer S6-1b also exhibited excellent selectivity, making it suitable for use in a complex biological environment. Furthermore, the aptamer can effectively target glioma cells for in vivo fluorescence imaging of tumors. The target type of aptamer S6-1b was identified as a cell membrane protein. Our work indicates that aptamer S6-1b has diagnostic and therapeutic potential to specifically deliver imaging or therapeutic agents to malignant gliomas.
中文翻译：
Cell-SELEX 生成的适体用于特异性靶向人胶质瘤细胞
最常见的原发性脑肿瘤是神经胶质瘤，它始于神经胶质细胞。尽管手术和放化疗技术取得了显着的进步，但恶性胶质瘤患者的预后和生存率仍然很差。对于神经胶质瘤的常规诊断，计算机断层扫描和磁共振成像主要依赖于解剖学变化，无法检测到恶性神经胶质瘤发展早期发生的细胞变化。因此，迫切需要找到有效的分子诊断工具来检测早期恶性胶质瘤。目前，基于细胞的指数富集配体系统进化（cell-SELEX）技术是获得能够区分不同类型细胞系分子特征的DNA或RNA适体的一种有效工具。使用 cell-SELEX，我们生成并表征了一种适体，称为 S6-1b，它可以区分神经胶质瘤细胞系 SHG44 和人星形胶质细胞之间的分子差异。分别在 4 和 37 °C 的条件下，适配体-细胞相互作用的解离常数均在低纳摩尔范围内测量。适配体S6-1b还表现出优异的选择性，使其适合在复杂的生物环境中使用。此外，该适配体可以有效地靶向神经胶质瘤细胞，用于肿瘤的体内荧光成像。适配体S6-1b的目标类型被鉴定为细胞膜蛋白。我们的工作表明适体 S6-1b 具有诊断和治疗潜力，可以特异性地将成像或治疗剂递送至恶性神经胶质瘤。
更新日期：2020-10-08
中文翻译：
Cell-SELEX 生成的适体用于特异性靶向人胶质瘤细胞
最常见的原发性脑肿瘤是神经胶质瘤，它始于神经胶质细胞。尽管手术和放化疗技术取得了显着的进步，但恶性胶质瘤患者的预后和生存率仍然很差。对于神经胶质瘤的常规诊断，计算机断层扫描和磁共振成像主要依赖于解剖学变化，无法检测到恶性神经胶质瘤发展早期发生的细胞变化。因此，迫切需要找到有效的分子诊断工具来检测早期恶性胶质瘤。目前，基于细胞的指数富集配体系统进化（cell-SELEX）技术是获得能够区分不同类型细胞系分子特征的DNA或RNA适体的一种有效工具。使用 cell-SELEX，我们生成并表征了一种适体，称为 S6-1b，它可以区分神经胶质瘤细胞系 SHG44 和人星形胶质细胞之间的分子差异。分别在 4 和 37 °C 的条件下，适配体-细胞相互作用的解离常数均在低纳摩尔范围内测量。适配体S6-1b还表现出优异的选择性，使其适合在复杂的生物环境中使用。此外，该适配体可以有效地靶向神经胶质瘤细胞，用于肿瘤的体内荧光成像。适配体S6-1b的目标类型被鉴定为细胞膜蛋白。我们的工作表明适体 S6-1b 具有诊断和治疗潜力，可以特异性地将成像或治疗剂递送至恶性神经胶质瘤。
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